Ranken R, White C F, Gottfried T G, Yonkovich S J, Blazek B E, Moss M S, Fee W E, Liu Y S
InTek Diagnostics, Inc., Burlingame, California 94010.
Cancer Res. 1987 Nov 1;47(21):5684-90.
Monoclonal antibody 17.13., derived from a fusion of splenocytes of a BALB/c mouse immunized with a surgically resected poorly differentiated human laryngeal recurrent squamous cell carcinoma (SCC) with mouse Sp2/0 cells, is an IgM-K which recognizes a cytoplasmic component of basal cells. Tissue sections of malignant and normal squamous epithelium, tumors of nonsquamous origin, and normal and malignant cytological specimens were tested with an immunoperoxidase assay. Seventy-nine of 81 (98%) SCC of the head and neck, 26 of 26 (100%) SCC of the cervical and female gynecological tract, 29 of 30 (97%) SCC of the lung, 19 of 19 (100%) SCC of the oral cavity, and 17 of 17 (100%) SCC-involved neck lymph nodes reacted strongly. Various carcinomas from breast, colon, ovary, and others were unreactive. In normal squamous epithelial tissues, monoclonal antibody 17.13. reacts only with basal cells but not the cells above the basal layers. Normal tissues from heart, liver, spleen, kidney, bladder, colon, ovary, stomach, pancreas, breast, lung, prostate, thyroid, and lymph nodes were unreactive with the exception of myoepithelial cells. Monoclonal antibody 17.13. may be useful in the diagnosis and management of SCC.
单克隆抗体17.13由用手术切除的低分化人喉复发性鳞状细胞癌（SCC）免疫的BALB/c小鼠的脾细胞与小鼠Sp2/0细胞融合而成，是一种IgM-K，可识别基底细胞的一种细胞质成分。采用免疫过氧化物酶测定法对恶性和正常鳞状上皮组织切片、非鳞状起源肿瘤以及正常和恶性细胞学标本进行检测。81例头颈部SCC中有79例（98%）、26例宫颈和女性生殖道SCC中有26例（100%）、30例肺部SCC中有29例（97%）、19例口腔SCC中有19例（100%）以及17例SCC累及的颈部淋巴结中有17例（100%）反应强烈。来自乳腺、结肠、卵巢等的各种癌均无反应。在正常鳞状上皮组织中，单克隆抗体17.13仅与基底细胞反应，而不与基底层以上的细胞反应。除肌上皮细胞外，心脏、肝脏、脾脏、肾脏、膀胱、结肠、卵巢、胃、胰腺、乳腺、肺、前列腺、甲状腺和淋巴结的正常组织均无反应。单克隆抗体17.13可能有助于SCC的诊断和管理。
