Nashida T, Imai A, Shimomura H
Department of Oral Biochemistry, Nippon Dental University, School of Dentistry, Niigata, Japan.
Arch Oral Biol. 1994 Jul;39(7):629-32. doi: 10.1016/0003-9969(94)90139-2.
Atrial natriuretic peptide (ANP), sodium nitroprusside and hydroxylamine increased cGMP accumulation in rat parotid acinar cells both in the presence and absence of forskolin but in a different manner. On the other hand, ANP decreased forskolin-stimulated cAMP accumulation, although sodium nitroprusside and hydroxylamine had no effect on cAMP accumulation. Amylase release stimulated by forskolin, dibutyryl-cAMP or isoproterenol was depressed by ANP, whereas sodium nitroprusside and hydroxylamine did not evoke the inhibition of forskolin-stimulated amylase release. These results suggest that the inhibition of cAMP accumulation and of amylase release by ANP were not mediated via cGMP produced by guanylate cyclase-A.
心房利钠肽（ANP）、硝普钠和羟胺在有无福斯高林的情况下均可增加大鼠腮腺腺泡细胞中cGMP的积累，但方式不同。另一方面，ANP可降低福斯高林刺激的cAMP积累，而硝普钠和羟胺对cAMP积累无影响。福斯高林、二丁酰-cAMP或异丙肾上腺素刺激的淀粉酶释放受到ANP的抑制，而硝普钠和羟胺并未引起对福斯高林刺激的淀粉酶释放的抑制。这些结果表明，ANP对cAMP积累和淀粉酶释放的抑制并非通过鸟苷酸环化酶-A产生的cGMP介导。
