Carboni E, Wojcik W J, Costa E
Neuropharmacology. 1985 Nov;24(11):1123-6. doi: 10.1016/0028-3908(85)90202-3.
The uptake of calcium (Ca++) into cerebellar granule cells in primary culture was increased by depolarizing the cells with either 60 mM KC1 or veratridine. Nitrendipine, at concentrations of 100 nM or greater, antagonized approximately 40 percent of the depolarization induced Ca++ uptake. The half maximal concentration of nitrendipine was 7nM. Furthermore, another dihydropyridine derivative, BAY K 8644 enhanced the uptake of Ca++ and in the presence of nitrendipine, this facilitation of Ca++ uptake was reduced. Thus, these data indicate the existence of voltage dependent Ca++ channels which are sensitive to dihydropyridines in primary cultures of cerebellar granule cells.
用60 mM氯化钾或藜芦碱使原代培养的小脑颗粒细胞去极化，可增加细胞对钙离子（Ca++）的摄取。硝苯地平浓度在100 nM及以上时，可拮抗约40%的去极化诱导的Ca++摄取。硝苯地平的半数最大浓度为7 nM。此外，另一种二氢吡啶衍生物BAY K 8644可增强Ca++的摄取，而在硝苯地平存在的情况下，这种对Ca++摄取的促进作用会减弱。因此，这些数据表明在小脑颗粒细胞的原代培养物中存在对二氢吡啶敏感的电压依赖性Ca++通道。
