• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

大肠杆菌启动子的有效转录起始速率与链解链特性之间的相关性。

Correlation between the rate of productive transcription initiation and the strand-melting property of Escherichia coli promoters.

作者信息

Tachibana H, Ishihama A

出版信息

Nucleic Acids Res. 1985 Dec 20;13(24):9031-42. doi: 10.1093/nar/13.24.9031.

DOI:10.1093/nar/13.24.9031
PMID:2417199
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318969/
Abstract

"Opening potential"s of DNA segments of about ten base pairs in length were calculated for eleven promoters of Escherichia coli using the thermodynamic parameter values [Gotoh and Tagashira (1981) Biopolymers 20, 1043-1058] for the stabilities of ten kinds of nearest neighbor base pair doublets in a melting reaction. They were compared with the strength of each promoter determined experimentally with a "mixed transcription" system [Kajitani and Ishihama (1983) Nucleic Acids Res. 11, 3873-3888]. A positive correlation was found between the calculated opening potential in the -9 to +3 region (+1 denotes the nucleotide position at which the transcription starts) and the rate of open complex formation.

摘要

利用十类相邻碱基对双链体在解链反应中的稳定性的热力学参数值[后藤和田代（1981年），《生物聚合物》20，1043 - 1058]，计算了大肠杆菌11个启动子中长度约为10个碱基对的DNA片段的“开放潜能”。将其与通过“混合转录”系统[梶谷和石滨（1983年），《核酸研究》11，3873 - 3888]实验测定的每个启动子的强度进行了比较。发现在 - 9至 + 3区域（+1表示转录起始的核苷酸位置）计算出的开放潜能与开放复合物形成速率之间存在正相关。

相似文献

1
Correlation between the rate of productive transcription initiation and the strand-melting property of Escherichia coli promoters.大肠杆菌启动子的有效转录起始速率与链解链特性之间的相关性。
Nucleic Acids Res. 1985 Dec 20;13(24):9031-42. doi: 10.1093/nar/13.24.9031.
2
Aromatic amino acids in region 2.3 of Escherichia coli sigma 70 participate collectively in the formation of an RNA polymerase-promoter open complex.大肠杆菌σ70因子2.3区域中的芳香族氨基酸共同参与RNA聚合酶-启动子开放复合物的形成。
J Mol Biol. 2000 Jun 23;299(5):1217-30. doi: 10.1006/jmbi.2000.3808.
3
A direct real-time spectroscopic investigation of the mechanism of open complex formation by T7 RNA polymerase.对T7 RNA聚合酶形成开放复合物机制的直接实时光谱研究。
Biochemistry. 1996 Dec 10;35(49):15715-25. doi: 10.1021/bi960729d.
4
The G+C-rich discriminator region of the tyrT promoter antagonises the formation of stable preinitiation complexes.tyrT启动子富含G+C的鉴别区域会拮抗稳定的起始前复合物的形成。
J Mol Biol. 2000 Jun 16;299(4):859-64. doi: 10.1006/jmbi.2000.3780.
5
In vitro studies of transcript initiation by Escherichia coli RNA polymerase. 1. RNA chain initiation, abortive initiation, and promoter escape at three bacteriophage promoters.大肠杆菌RNA聚合酶转录起始的体外研究。1. 三种噬菌体启动子处的RNA链起始、流产起始和启动子逃逸
Biochemistry. 2003 Apr 8;42(13):3777-86. doi: 10.1021/bi026954e.
6
Mechanism of regulation of transcription initiation by ppGpp. I. Effects of ppGpp on transcription initiation in vivo and in vitro.ppGpp对转录起始的调控机制。I. ppGpp对体内和体外转录起始的影响。
J Mol Biol. 2001 Jan 26;305(4):673-88. doi: 10.1006/jmbi.2000.4327.
7
Promoter selectivity of Escherichia coli RNA polymerase: effect of base substitutions in the promoter -35 region on promoter strength.大肠杆菌RNA聚合酶的启动子选择性：启动子 -35区域碱基替换对启动子强度的影响。
Nucleic Acids Res. 1990 Dec 25;18(24):7367-72. doi: 10.1093/nar/18.24.7367.
8
Thermal energy requirement for strand separation during transcription initiation: the effect of supercoiling and extended protein DNA contacts.转录起始过程中链分离所需的热能：超螺旋和扩展的蛋白质-DNA接触的影响
Nucleic Acids Res. 1994 Sep 25;22(19):3840-5. doi: 10.1093/nar/22.19.3840.
9
Mechanism of regulation of transcription initiation by ppGpp. II. Models for positive control based on properties of RNAP mutants and competition for RNAP.ppGpp对转录起始的调控机制。II. 基于RNA聚合酶突变体特性和RNA聚合酶竞争的正调控模型。
J Mol Biol. 2001 Jan 26;305(4):689-702. doi: 10.1006/jmbi.2000.4328.
10
On the base sequences of the promoters in transcription initiation.关于转录起始中启动子的碱基序列。
Biophys Chem. 1992 Aug;44(1):61-70. doi: 10.1016/0301-4622(92)85035-3.

引用本文的文献

1
Enhancing the Sweetness of Yoghurt through Metabolic Remodeling of Carbohydrate Metabolism in Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus.通过嗜热链球菌和德氏保加利亚乳杆菌碳水化合物代谢的代谢重塑提高酸奶甜度
Appl Environ Microbiol. 2016 May 31;82(12):3683-3692. doi: 10.1128/AEM.00462-16. Print 2016 Jun 15.
2
The role of TFIIB-RNA polymerase II interaction in start site selection in yeast cells.TFIIB与RNA聚合酶II的相互作用在酵母细胞起始位点选择中的作用。
Nucleic Acids Res. 2002 Jul 15;30(14):3078-85. doi: 10.1093/nar/gkf422.
3
A mutant RNA polymerase that forms unusual open promoter complexes.一种形成异常开放启动子复合物的突变型RNA聚合酶。
Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13481-6. doi: 10.1073/pnas.94.25.13481.
4
Analysis of E. coli promoter sequences.大肠杆菌启动子序列分析。
Nucleic Acids Res. 1987 Mar 11;15(5):2343-61. doi: 10.1093/nar/15.5.2343.
5
Mutations of the Escherichia coli lacUV5 promoter resulting in increased expression in Bacillus subtilis.大肠杆菌lacUV5启动子的突变导致其在枯草芽孢杆菌中的表达增加。
Mol Gen Genet. 1987 Oct;209(3):467-74. doi: 10.1007/BF00331151.
6
Nucleotide sequence of the kanamycin resistance determinant of the pneumococcal transposon Tn1545: evolutionary relationships and transcriptional analysis of aphA-3 genes.肺炎球菌转座子Tn1545卡那霉素抗性决定簇的核苷酸序列：aphA-3基因的进化关系及转录分析
Mol Gen Genet. 1987 May;207(2-3):509-13. doi: 10.1007/BF00331623.
7
A novel method for promoter search enhanced by function-specific subgrouping of promoters--developed and tested on E.coli system.一种通过启动子功能特异性亚分组增强的新型启动子搜索方法——在大肠杆菌系统上开发并测试。
Nucleic Acids Res. 1989 Jun 26;17(12):4799-815. doi: 10.1093/nar/17.12.4799.
8
DNA supercoiling and temperature shift affect the promoter activity of the Escherichia coli rpoH gene encoding the heat-shock sigma subunit of RNA polymerase.
Mol Gen Genet. 1989 Jan;215(2):185-9. doi: 10.1007/BF00339716.
9
Promoter selectivity of Escherichia coli RNA polymerase: effect of base substitutions in the promoter -35 region on promoter strength.大肠杆菌RNA聚合酶的启动子选择性：启动子 -35区域碱基替换对启动子强度的影响。
Nucleic Acids Res. 1990 Dec 25;18(24):7367-72. doi: 10.1093/nar/18.24.7367.

本文引用的文献

1
Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.根据热变性温度确定脱氧核糖核酸的碱基组成
J Mol Biol. 1962 Jul;5:109-18. doi: 10.1016/s0022-2836(62)80066-7.
2
Locations of frequently opening regions on natural DNAs and their relation to functional loci.天然DNA上频繁开放区域的位置及其与功能位点的关系。
Biopolymers. 1981 May;20(5):1043-58. doi: 10.1002/bip.1981.360200514.
3
Ligand-induced melting reaction of specific-sequence DNA molecules.
Biopolymers. 1982 Sep;21(9):1873-85. doi: 10.1002/bip.360210914.
4
The complete nucleotide sequence of the tryptophan operon of Escherichia coli.大肠杆菌色氨酸操纵子的完整核苷酸序列。
Nucleic Acids Res. 1981 Dec 21;9(24):6647-68. doi: 10.1093/nar/9.24.6647.
5
Transcription of the E. coli tufB gene: cotranscription with four tRNA genes and inhibition by guanosine-5'-diphosphate-3'-diphosphate.大肠杆菌tufB基因的转录：与四个tRNA基因共转录以及受鸟苷-5'-二磷酸-3'-二磷酸抑制
Mol Gen Genet. 1981;183(1):13-9. doi: 10.1007/BF00270131.
6
The nucleotide sequence of tufB and four nearby tRNA structural genes of Escherichia coli.大肠杆菌tufB及四个相邻tRNA结构基因的核苷酸序列。
Gene. 1980 Dec;12(1-2):33-9. doi: 10.1016/0378-1119(80)90013-x.
7
Spacer mutations in the lac ps promoter.乳糖操纵子启动子中的间隔突变
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1069-72. doi: 10.1073/pnas.79.4.1069.
8
Mutation-induced changes in RNA polymerase-lac ps promoter interactions.突变诱导的RNA聚合酶与乳糖操纵子启动子相互作用的变化。
J Biol Chem. 1982 Dec 10;257(23):13924-9.
9
Correlation of Tm and sequence of DNA duplexes with delta H computed by an improved empirical potential method.通过改进的经验势方法计算的DNA双链体的熔解温度（Tm）与序列及焓变（ΔH）的相关性。
Biopolymers. 1983 Aug;22(8):1979-2000. doi: 10.1002/bip.360220811.
10
In vitro comparison of initiation properties of bacteriophage lambda wild-type PR and x3 mutant promoters.噬菌体λ野生型PR启动子和x3突变体启动子起始特性的体外比较。
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6381-5. doi: 10.1073/pnas.77.11.6381.