Guangdong Provincial Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, China.
Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation Department of Obstetrics and Gynecology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China.
Mediators Inflamm. 2018 Jun 5;2018:8354902. doi: 10.1155/2018/8354902. eCollection 2018.
To profile the dynamic changes of immune responses for infection, 3 cynomolgus monkeys were experimentally infected with by intratracheal inhalation of 1 × 10 CFU bacteria per monkey. Every 2 to 4 weeks, tuberculin skin testings (TSTs) were performed and blood samples were collected for immunoassay. Multiple cytokines in a single sample were measured by Luminex xMAP technologies. IgM and IgA were detected by double-antibody sandwich ELISA. IgG against PPD and 11 proteins were detected by using of indirect ELISA. At week 16, all animals were euthanized for necropsy and histological analysis. Positivities of TSTs emerged from week 2 to 6 postinfection. Leukocyte counts and T lymphocyte subsets experienced moderate increases. Among 44 kinds of cytokines, 36 kinds of them showed increases of different dynamic types and 8 kinds of them showed no specific changes. Total IgM and IgA showed a transient increase at an early infection stage. Positivities of specific IgM and IgA emerged as early as week 2 postinfection. All animals showed positive IgG against PPD and negative IgG responses to 38 kDa, MPT64L, TB16.3, 16 kDa, U1, and MTB81 antigens during the infection period. IgG against ESAT-6, CFP10, CFP10-ESAT-6, Ag85b, and 14 kDa antigens reached positive levels. The IgG avidities of PPD, ESAT-6, CFP10-ESAT-6, and Ag85b were all above 50 percent. In conclusion, the data indicate that infection in monkeys can induce positivities of TSTs, increases of multiple cytokines, and cross-reactive antibody responses to antigens.
为了描绘感染后免疫反应的动态变化，3 只食蟹猴通过气管内吸入 1×10 CFU 细菌/只的方式进行实验性感染。每 2 到 4 周进行一次结核菌素皮肤试验（TST），并采集血液样本进行免疫测定。通过 Luminex xMAP 技术在单个样本中测量多种细胞因子。通过双抗体夹心 ELISA 检测 IgM 和 IgA。使用间接 ELISA 检测针对 PPD 和 11 种蛋白质的 IgG。在第 16 周，所有动物均被安乐死进行尸检和组织学分析。TST 阳性从感染后第 2 周到第 6 周出现。白细胞计数和 T 淋巴细胞亚群经历了中度增加。在 44 种细胞因子中，36 种表现出不同动态类型的增加，8 种没有特定变化。总 IgM 和 IgA 在早期感染阶段短暂增加。特异性 IgM 和 IgA 的阳性早在感染后第 2 周就出现了。在感染期间，所有动物均对 PPD 呈阳性 IgG 反应，对 38 kDa、MPT64L、TB16.3、16 kDa、U1 和 MTB81 抗原呈阴性 IgG 反应。针对 ESAT-6、CFP10、CFP10-ESAT-6、Ag85b 和 14 kDa 抗原的 IgG 反应均达到阳性水平。PPD、ESAT-6、CFP10-ESAT-6 和 Ag85b 的 IgG 亲和力均高于 50%。综上所述，数据表明，猴感染可引起 TST 阳性、多种细胞因子增加和针对 抗原的交叉反应性抗体反应。
