细胞内钙调节氯化物分泌上皮中的基底外侧钾通道。
Intracellular calcium regulates basolateral potassium channels in a chloride-secreting epithelium.
作者信息
Welsh M J, McCann J D
出版信息
Proc Natl Acad Sci U S A. 1985 Dec;82(24):8823-6. doi: 10.1073/pnas.82.24.8823.
Abstract
The two individual cell membranes of epithelia are functionally coupled, so that changes in apical membrane conductance are paralleled by changes in basolateral K+ conductance. However, the signal that regulates basolateral K+ conductance, thereby coupling the two membranes, is unknown. We tested the hypothesis that the cellular calcium concentration, [Ca2+]c, may regulate basolateral K+ conductance in canine tracheal epithelium, a Cl- -secreting epithelium that shows marked membrane coupling. Three findings support the hypothesis. First, the intracellular Ca2+ antagonist 8-(diethylamino)octyl 3,4,5-trimethoxybenzoate hydrochloride (TMB-8) attenuated the secretory response. Second, the secretagogue epinephrine increased [Ca2+]c, as measured with quin-2. Third, we found a K+ channel that was activated by Ca2+ on the cytosolic side of the membrane. Thus, cytosolic Ca2+ regulates the basolateral K+ conductance and may be the signal responsible for functional coupling of the two cell membranes.
摘要
上皮细胞的两个独立细胞膜在功能上是耦合的，因此顶端膜电导的变化与基底外侧K⁺电导的变化是平行的。然而，调节基底外侧K⁺电导从而使两个膜耦合的信号尚不清楚。我们检验了这样一个假设，即细胞内钙浓度[Ca²⁺]c可能调节犬气管上皮细胞的基底外侧K⁺电导，犬气管上皮细胞是一种分泌Cl⁻的上皮细胞，表现出明显的膜耦合。有三个发现支持了这一假设。第一，细胞内Ca²⁺拮抗剂盐酸8-（二乙氨基）辛基3,4,5-三甲氧基苯甲酸酯（TMB-8）减弱了分泌反应。第二，用喹啉-2测量，促分泌剂肾上腺素增加了[Ca²⁺]c。第三，我们发现了一种在膜胞质侧被Ca²⁺激活的K⁺通道。因此，胞质Ca²⁺调节基底外侧K⁺电导，可能是两个细胞膜功能耦合的信号。
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