Roth K A, Makk G, Beck O, Faull K, Tatemoto K, Evans C J, Barchas J D
Regul Pept. 1985 Nov 7;12(3):185-99. doi: 10.1016/0167-0115(85)90060-6.
Using an antiserum directed at the COOH-terminus of tachykinins, we have examined postmortem tissue from two cases of metastatic ileal carcinoid for the presence of tachykinin-like immunoreactivity. The vast majority of the immunoreactive tachykinin-like material eluted from a Sephadex G-50 column as two peaks at positions corresponding to molecular weights of 1300 and 850. The 1300 dalton peak was resolved by reverse-phase-HPLC into two components which by Edman sequencing, amino acid analysis, and fast atom bombardment (FAB)-mass spectrometry criteria, were identified as substance P and substance K. The 850 dalton peak was also resolved on RP-HPLC into two peaks which were resistant to Edman degradation but from amino acid analysis and FAB-mass spectrometry criteria were identified as pyro-Glu-substance P 5-11 and oxidized pyro-Glu-substance P 5-11. In control experiments substance P 5-11 was converted to pyro-Glu-substance P 5-11 during the extraction procedure. Both tumors also contained a minor immunoreactive peak which eluted from a Sephadex G-50 sizing column at a position corresponding to a molecular weight of 4000 which probably represents neuropeptide K. These results suggest that beta-preprotachykinin is preferentially expressed in carcinoid tumors and that substance K may also play a role in the carcinoid syndrome.
我们使用一种针对速激肽COOH末端的抗血清，检查了两例转移性回肠类癌的尸检组织中是否存在速激肽样免疫反应性物质。从Sephadex G - 50柱上洗脱下来的绝大多数免疫反应性速激肽样物质以两个峰的形式出现，其位置对应于分子量为1300和850。1300道尔顿的峰通过反相高效液相色谱法（RP - HPLC）分离为两个组分，根据埃德曼测序、氨基酸分析和快原子轰击（FAB）质谱标准，鉴定为P物质和K物质。850道尔顿的峰在RP - HPLC上也分离为两个峰，它们对埃德曼降解有抗性，但根据氨基酸分析和FAB质谱标准，鉴定为焦谷氨酸 - P物质5 - 11和氧化焦谷氨酸 - P物质5 - 11。在对照实验中，P物质5 - 11在提取过程中转化为焦谷氨酸 - P物质5 - 11。这两个肿瘤还含有一个较小的免疫反应性峰，从Sephadex G - 50分子筛柱上洗脱的位置对应于分子量4000，可能代表神经肽K。这些结果表明β - 前速激肽原在类癌肿瘤中优先表达，并且K物质可能在类癌综合征中也起作用。
