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茄子(茄科)随机扩增多态性 DNA 变异。

Random amplified polymorphic DNA variation in the eggplant, Solanum melongena L. (Solanaceae).

机构信息

N.F.P.T.C.R., National Bureau of Plant Genetic Resources, Pusa Campus, 110012, New Delhi, India.

出版信息

Theor Appl Genet. 1995 May;90(6):767-70. doi: 10.1007/BF00222010.

Abstract

RAPD analysis was carried out on 52 accessions of Solanum melongena (eggplant) and related weedy forms known as "insanum". Twenty-two primers amplified 130 fragments. Solanum melongena exhibited 117 of the fragments, all of which were also present in insanum. Insanum displayed an additional 13 fragments not found in S. melongena. Overall, the insanum accessions were more diverse than those of S. melongena. The calculated similarity between them was 0.947. The RAPD results were closely concordant with the results of an electrophoretic isozyme survey performed on the same accessions. The concordance of the results shows that even though S. melongena and insanum are highly diverse morphologically, it is no longer appropriate to distinguish them taxonomically.

摘要

RAPD 分析对 52 份茄子(茄子)和相关杂草形式的访问进行了分析,这些杂草被称为“insanum”。22 个引物扩增了 130 个片段。茄子显示了 117 个片段,所有这些片段也存在于 insanum 中。Insanum 显示了 13 个不在茄子中发现的额外片段。总体而言, insanum 访问者比 S. melongena 访问者更具多样性。它们之间计算出的相似性为 0.947。RAPD 结果与对同一访问者进行的电泳同工酶调查的结果非常一致。结果的一致性表明,即使茄子和 insanum 在形态上高度多样化,从分类学上区分它们已经不再合适。

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