Lehrstuhl für Pflanzenbau und Pflanzenzuechtung, Technical University Munich, 85350, Freising-Weihenstephan, Germany.
Theor Appl Genet. 1995 Apr;90(5):601-6. doi: 10.1007/BF00222121.
RFLP markers for the wheat powdery mildew resistance genes Pm1 and Pm2 were tagged by means of near-isogenic lines. The probe Whs178 is located 3 cM from the Pm1 gene. For the powdery mildew resistance gene Pm2, two markers were identified. The linkage between the Pm2 resistance locus and one of these two probes was estimated to be 3 cM with a F2 population. Both markers can be used to detect the presence of the corresponding resistance gene in commercial cultivars. "Bulked segregant analysis" was applied to identify linkage disequillibrium between the resistance gene Pm18 and the abovementioned marker, which was linked to this locus at a distance of 4 cM. Furthermore, the RAPD marker OPH-111900 (5'-CTTCCGCAGT-3') was selected with pools created from a population segregating for the resistance of 'Trigo BR 34'. The RAPD marker was mapped about 13 cM from this resistance locus.
通过近等基因系，为小麦白粉病抗性基因 Pm1 和 Pm2 标记了 RFLP 标记。探针 Whs178 位于 Pm1 基因 3cM 处。对于白粉病抗性基因 Pm2，鉴定出了两个标记。用 F2 群体估计 Pm2 抗性基因座与这两个探针之一之间的连锁关系为 3cM。这两个标记都可用于检测商业品种中相应抗性基因的存在。“混池分离分析法”被应用于鉴定抗性基因 Pm18 与上述标记之间的连锁不平衡，该标记与该基因座的距离为 4cM。此外，选择了来自“Trigo BR 34”抗性分离群体的池，选择了 RAPD 标记 OPH-111900（5'-CTTCCGCAGT-3'）。该 RAPD 标记位于该抗性基因座约 13cM 处。
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