Genome. 1994 Oct;37(5):871-5. doi: 10.1139/g94-123.
Near-isogenic lines (NILs) and their recurrent parent Chancellor (Cc) were used to identify restriction fragment length polymorphic markers linked to powdery mildew (Blumeria graminis (DC.) E.O. Speer f.sp. tritici) resistance genes Pm1, Pm2, Pm3, and Pm4 in wheat (Triticum aestivum L. em. Thell). By mapping these polymorphic markers in F2 progenies from crosses of the NILs with Cc, it was found that Pm1 cosegregated with a polymorphic locus detected by DNA probe CDO347; Pm2 was linked to a locus detected by probe BCD1871 with a distance of 3.5 cM; Pm3b was linked to a locus detected by probe BCD1434 with a distance of 1.3 cM; Pm4a cosegregated with Xbcd1231-2A(2) and Xcdo678-2A, and was closely flanked by Xbcd1231-2A(1) and Xbcd292-2A both with a distance of 1.5 cM. Aneuploid mapping of these markers indicated that locus Xcdo347-7A is on 7AL, Xbcd1871-5D on 5DS, Xbcd1434-1A on 1AS, and loci Xbcd292-2A and Xcdo678-2A are on 2AL. The same polymorphic fragments detected in the Pm3b NIL by Xbcd1434-1A were found in Pm3a NIL using several enzyme digestions.
近等基因系（NILs）及其轮回亲本 Chancellor（Cc）被用于鉴定与小麦（Triticum aestivum L. em. Thell）抗白粉病基因 Pm1、Pm2、Pm3 和 Pm4 连锁的限制性片段长度多态性标记。通过在 NILs 与 Cc 的杂交 F2 后代中对这些多态性标记进行作图，发现 Pm1 与 DNA 探针 CDO347 检测到的多态性位点共分离；Pm2 与探针 BCD1871 检测到的位点连锁，距离为 3.5 cM；Pm3b 与探针 BCD1434 检测到的位点连锁，距离为 1.3 cM；Pm4a 与 Xbcd1231-2A(2)和 Xcdo678-2A 共分离，与 Xbcd1231-2A(1)和 Xbcd292-2A 紧密连锁，距离均为 1.5 cM。这些标记的非整倍体作图表明，位点 Xcdo347-7A 位于 7AL 上，Xbcd1871-5D 位于 5DS 上，Xbcd1434-1A 位于 1AS 上，位点 Xbcd292-2A 和 Xcdo678-2A 位于 2AL 上。在 Pm3b NIL 中用 Xbcd1434-1A 检测到的相同多态性片段，在 Pm3a NIL 中用几种酶消化也检测到。
