Cereals Research Department, John Innes Centre, Norwich Research Park, NR4 7UJ, Colney, UK.
Theor Appl Genet. 1995 Apr;90(5):740-5. doi: 10.1007/BF00222142.
Oligonucleotide primers were developed to detect the presence of four rye sequences using a PCR assay. These assays give a rye-specific signal from wheat DNA template which contains various rye chromosomes or chromosome segments. The sequences identified were associated with the nucleolar organiser region, the 5S-Rrna-R1 locus, the telomere, and a widely dispersed, rye-specific repetitive element Ris-1. The primers amplified from the well-established loci Nor-R1 and 5S-Rrna-R1 on rye chromosome arm 1RS, and also located a 5s-Rrna locus on chromosome 3R. The telomere-associated sequence was present on every rye chromosome, and was also present, at a low copy number, in both wheat and barley. These assays will be particularly useful for introgression programmes aimed at reducing the rye content of the 1BL.1RS wheat-rye translocation. When multiplexed, the primers will enable a rapid, simultaneous assay for a number of distinct rye loci, which can be derived from a small portion of mature endosperm tissue.
寡核苷酸引物被开发出来，用于使用 PCR 检测四种黑麦序列的存在。这些检测方法从含有各种黑麦染色体或染色体片段的小麦 DNA 模板中产生黑麦特异性信号。鉴定出的序列与核仁组织者区域、5S-Rrna-R1 基因座、端粒以及广泛分散的黑麦特异性重复元件 Ris-1 有关。这些引物从黑麦染色体 1RS 上的已建立好的 Nor-R1 和 5S-Rrna-R1 基因座扩增，也定位到了 3R 染色体上的一个 5s-Rrna 基因座。与端粒相关的序列存在于每一条黑麦染色体上，在小麦和大麦中也以低拷贝数存在。这些检测方法将特别有助于旨在降低 1BL.1RS 小麦-黑麦易位体中黑麦含量的导入计划。当多重检测时，这些引物将能够快速、同时检测多个不同的黑麦基因座，这些基因座可以从小麦成熟胚乳组织的一小部分中获得。
