Genome. 1995 Jun;38(3):623-6. doi: 10.1139/g95-079.
In situ hybridization was used to physically map the 5S rRNA multigene family in three selected lines of hexaploid triticale and five lines of diploid rye. Using this technique, evidence for a new locus on the 3RS arm of the three triticale lines was first obtained, as well as confirmation of the presence of 5S rRNA loci on wheat and rye chromosomes of homoeologous groups 1 and 5. The new locus on the 3RS arm was confirmed in two lines of rye, Secale cereale L., although it was not present in the other rye varieties studied. We propose that the new 5S rRNA locus be referred to as 5SDna-R3.
原位杂交技术被用于对六倍体小黑麦的三个选定品系和五倍体黑麦的五个品系中的 5S rRNA 多基因家族进行物理作图。使用该技术，首次在三个小黑麦品系的 3RS 臂上获得了一个新基因座的证据，同时还证实了小麦和黑麦同源群 1 和 5 上存在 5S rRNA 基因座。该新基因座在两个黑麦品系中得到了确认，尽管在其他研究的黑麦品种中不存在。我们建议将这个新的 5S rRNA 基因座称为 5SDna-R3。
