Bera Tushar Kanti, Ali Kazi Monjur, Jana Kishalay, Ghosh Abhinandan, Ghosh Debidas
Department of Bio-Medical Laboratory Science and Management, (UGC Innovative Funded Department), Vidyasagar University, West Bengal, India ; Department of Pharmaceutical Division, Southern Health Improvement Samity (SHIS), Bhangar, South 24 Paraganas, West Bengal, India.
Pharmacognosy Res. 2013 Oct;5(4):277-85. doi: 10.4103/0974-8490.118840.
Psoralea corylifolia (Somraji) and Trigonella foenum-graecum L. (Methi), important medicinal plants widely used in India as folk medicine. Local people of West Bengal traditionally used the seeds of these plants to cure diabetes.
Present study was designed to investigate the antidiabetic efficacy of aqueous extract of seeds of these plants in separate or in composite manner in streptozotocin (STZ)-induced diabetic rat.
Diabetes was induced by intramuscular injection of STZ at the dose of 40 mg/ml of citrate buffer/kg body weight. Fasting blood glucose (FBG), glyclated hemoglobin (HbA1C) and activities of hexokinase, glucose-6-phosphate dehydrogenase and glucose-6-phosphatase of liver in experimental animals were assessed. Hyperlipidemic state developed in the experimental diabetic rat was assessed by measuring the levels of total cholesterol, triglyceride, and lipoproteins in serum.
There was significant increased in the levels of FBG, HbA1C and lipid profiles along with diminution (P < 0.001) in the activities of hepatic hexokinase, glucose-6-phosphate dehydrogenase and elevation in glucose-6-phosphatase in diabetic control animals in respect to the untreated control. Significant recovery (P < 0.05) in the activities of above mentioned enzymes along with the correction in the levels of FBG, HbA1C and serum lipid profiles were noted towards the control level after the treatment of composite extract (i.e. 100 mg of Somraji: 100 mg of Methi, total 200 mg/kg body weight) than the individual extract (i.e. 200 mg of Somraji or 200 mg of Methi, per kg body weight) treatment.
Results suggest that composite extract of above plant parts has more potent antidiabetic efficacy than the individual extract.
补骨脂（索姆拉吉）和胡芦巴（甲硫氨酸）是印度广泛用作民间药物的重要药用植物。西孟加拉邦的当地人传统上使用这些植物的种子来治疗糖尿病。
本研究旨在调查这些植物种子的水提取物单独或复合使用对链脲佐菌素（STZ）诱导的糖尿病大鼠的抗糖尿病功效。
通过肌肉注射剂量为40mg/ml柠檬酸盐缓冲液/千克体重的STZ诱导糖尿病。评估实验动物的空腹血糖（FBG）、糖化血红蛋白（HbA1C）以及肝脏中己糖激酶、葡萄糖-6-磷酸脱氢酶和葡萄糖-6-磷酸酶的活性。通过测量血清中总胆固醇、甘油三酯和脂蛋白的水平来评估实验性糖尿病大鼠中出现的高脂血症状态。
与未治疗的对照组相比，糖尿病对照动物的FBG、HbA1C水平和脂质谱显著升高，同时肝脏己糖激酶、葡萄糖-6-磷酸脱氢酶的活性降低（P<0.001），葡萄糖-6-磷酸酶升高。与单独提取物（即每千克体重200mg索姆拉吉或200mg甲硫氨酸）治疗相比，复合提取物（即100mg索姆拉吉：100mg甲硫氨酸，总共200mg/kg体重）治疗后，上述酶的活性显著恢复（P<0.05），同时FBG、HbA1C水平和血清脂质谱恢复到对照水平。
结果表明，上述植物部位的复合提取物比单独提取物具有更强的抗糖尿病功效。
