Joshi Deval V, Patil Rajesh R, Naik Suresh R
Department of Pharmacology, Sinhgad Institute of Pharmaceutical Science , Lonavala, Pune, Maharashtra , India.
Pharm Biol. 2015 Feb;53(2):201-11. doi: 10.3109/13880209.2014.913296. Epub 2014 Oct 23.
The herb fenugreek, Trigonella foenum-graecum Linn (Fabaceae), seeds have been traditionally used in the treatment of diabetes but its effect on oxidative stress and pro-inflammatory cytokines in the improvement of exocrine function of diabetes has not been studied. The effect of hydroalcoholic extract of Trigonella foenum-graecum seeds (HEF) on alloxan-induced type-II diabetic rat model was investigated.
Effect of HEF (500, 1000, and 2000 mg/kg), glimepiride (4 mg/kg), and combination of HEF (500 mg/kg) + glimepiride (2 mg/kg), on alloxan-induced diabetic rats was evaluated by assaying (blood glucose, serum protein, glycosylated hemoglobin, muscle and liver glycogen, glucose uptake by diaphragm, liver glucose transport, serum pancreatic enzymes (α-amylase, lipase), pro-inflammatory cytokines (TNF-α, IL-6), antioxidant enzymes [glutathione (GSH), superoxide dismutase (SOD)], lipid peroxides (liver and pancreas), and histoarchitecture (liver, pancreas).
Treatment with HEF (at different doses), glimepiride, and HEF + glimepiride increased body weight and glucose uptake, reduced plasma glucose, glycosylated hemoglobin, liver glucose transport, pro-inflammatory cytokines, pancreatic enzymes and restored depleted glycogen (muscle, liver) and total protein significantly (p < 0.01) and dose dependently, including prevention of lipid peroxidation and restoration of GSH and SOD (liver and pancreas). Treatment with HEF + glimepiride potentiated hypoglycemic activity of glimepiride. Histoarchitecture of liver and pancreas showed marked improvement.
Present experimental findings suggest that HEF possesses promising hypoglycemic activity, presumably by amelioration of oxidative stress and pro-inflammatory cytokines. HEF may be useful as an adjuvant with clinically effective antidiabetic drugs in the management of type-II diabetes.
葫芦巴，即胡芦巴属植物胡芦巴（豆科）的种子，传统上用于治疗糖尿病，但其对氧化应激和促炎细胞因子在改善糖尿病外分泌功能方面的作用尚未得到研究。本研究考察了胡芦巴种子水醇提取物（HEF）对四氧嘧啶诱导的II型糖尿病大鼠模型的影响。
通过检测（血糖、血清蛋白、糖化血红蛋白、肌肉和肝糖原、膈肌葡萄糖摄取、肝脏葡萄糖转运、血清胰酶（α-淀粉酶、脂肪酶）、促炎细胞因子（TNF-α、IL-6）、抗氧化酶[谷胱甘肽（GSH）、超氧化物歧化酶（SOD）]、脂质过氧化物（肝脏和胰腺）以及组织结构（肝脏、胰腺），评估HEF（500、1000和2000mg/kg）、格列美脲（4mg/kg）以及HEF（500mg/kg）+格列美脲（2mg/kg）组合对四氧嘧啶诱导的糖尿病大鼠的影响。
HEF（不同剂量）、格列美脲以及HEF+格列美脲治疗可增加体重和葡萄糖摄取，降低血糖、糖化血红蛋白、肝脏葡萄糖转运、促炎细胞因子、胰酶，并显著（p<0.01）且剂量依赖性地恢复消耗的糖原（肌肉、肝脏）和总蛋白，包括预防脂质过氧化以及恢复肝脏和胰腺中的GSH和SOD。HEF+格列美脲治疗增强了格列美脲的降血糖活性。肝脏和胰腺的组织结构显示出明显改善。
目前的实验结果表明，HEF具有良好的降血糖活性，可能是通过改善氧化应激和促炎细胞因子实现的。HEF可能作为辅助药物与临床有效的抗糖尿病药物联合用于II型糖尿病的治疗。
