Petrukhin K E, Broude N E, Arsenian S G, Grishin A V, Dzhandzhurgazian K N
Bioorg Khim. 1985 Dec;11(12):1607-13.
Messenger RNA, coding for the alpha-subunit of the Na+, K+-ATPase, was isolated from outer medulla of pig kidney. Within 25S-26S region the mRNA yields a band of specific hybridization with three oligonucleotide probes synthesized according to data on structures of three peptides isolated from the tryptic hydrolysate of the protein. Translation of the enriched poly(A+)-fraction of RNA in Xenopus laevis oocytes followed by the immunochemical identification of the products confirmed the presence of RNA coding for the desired protein. This RNA preparation was used for synthesis and cloning of double stranded cDNA.
编码钠钾ATP酶α亚基的信使核糖核酸从猪肾外髓质中分离得到。在25S - 26S区域内，该信使核糖核酸与根据从该蛋白质胰蛋白酶水解产物中分离出的三种肽的结构数据合成的三种寡核苷酸探针产生特异性杂交带。将富含多聚腺苷酸（poly(A+)）的RNA组分在非洲爪蟾卵母细胞中进行翻译，随后对产物进行免疫化学鉴定，证实了编码所需蛋白质的RNA的存在。这种RNA制剂用于双链互补脱氧核糖核酸（cDNA）的合成和克隆。
