[猪肾Na⁺,K⁺-ATP酶β亚基的一级结构。II. 逆转录、mRNA克隆、对应于基因结构区域的完整核苷酸序列]

[Primary structure of the beta-subunit of Na+,K+-ATPase from the swine kidney. II. Reverse transcription, cloning of mRNA, complete nucleotide sequence corresponding to the structural region of the gene].

作者信息

Broude N E, Monastyrskaia G S, Petrukhin K E, Grishin A V, Kiiatkin N I

出版信息

Bioorg Khim. 1987 Jan;13(1):14-9.

Abstract

mRNA coding for beta-subunit of Na+, K+-ATPase from pig kidney is about 24-25S as deduced from the hybridization pattern of poly (A)+-RNA with two synthetic oligonucleotides structurally corresponding to two peptides isolated from the tryptic hydrolyzate of beta-subunit. Cloning of cDNA allowed to determine the complete structure of the gene and to deduce the amino acid sequence of beta-subunit of Na+, K+-ATPase. The beta-subunit contains 302 amino acid residues and the protein is not processed at the N-nor at the C-terminus.

摘要

从猪肾钠钾ATP酶β亚基的多聚腺苷酸RNA与两个结构上对应于从β亚基胰蛋白酶水解产物中分离出的两种肽的合成寡核苷酸的杂交模式推断，编码该亚基的信使核糖核酸约为24-25S。互补DNA的克隆使得能够确定该基因的完整结构，并推断出钠钾ATP酶β亚基的氨基酸序列。β亚基含有302个氨基酸残基，该蛋白质在N端和C端均未经过加工。

相似文献

1

[Primary structure of the beta-subunit of Na+,K+-ATPase from the swine kidney. II. Reverse transcription, cloning of mRNA, complete nucleotide sequence corresponding to the structural region of the gene].[猪肾Na⁺,K⁺-ATP酶β亚基的一级结构。II. 逆转录、mRNA克隆、对应于基因结构区域的完整核苷酸序列]

Bioorg Khim. 1987 Jan;13(1):14-9.

2

[Primary structure of the alpha subunit of Na+,K+-ATPase. II. Isolation, reverse transcription and cloning of messenger RNA].[钠钾ATP酶α亚基的一级结构。II. 信使核糖核酸的分离、逆转录及克隆]

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[Nucleotide sequence of cDNA and primary structure of the NA+,K+-ATPase beta-subunit from the swine kidney].[猪肾Na⁺,K⁺-ATP酶β亚基的cDNA核苷酸序列及一级结构]

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6

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Nature. 1986;321(6068):429-31. doi: 10.1038/321429a0.

7

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J Neurosci Res. 1989 May;23(1):9-20. doi: 10.1002/jnr.490230103.

8

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10

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