İnce İlker, Akar İlker, Arıcı Akgül
Department of Cardiovascular Surgery, Dışkapı Training and Research Hospital, Ankara, Turkey.
Department of Cardiovascular Surgery, Gaziosmanpaşa University Faculty of Medicine, Tokat, Turkey.
Turk Gogus Kalp Damar Cerrahisi Derg. 2020 Apr 22;28(2):274-281. doi: 10.5606/tgkdc.dergisi.2020.18905. eCollection 2019 Jun.
In this experimental study, we aimed to investigate the efficacy of edaravone on renal injury due to acute lower limb ischemia/reperfusion in a rat model.
Between June 2015 and August 2015, a total of 40 male Wistar rats were used in this study. The rats were randomly divided into the sham, ischemia/reperfusion, edaravone, and solvent groups (n=10 in each). The infrarenal abdominal aorta was clamped for 120 min and was, then, reperfused for 120 min after clamp removal. Edaravone was administered intravenously 30 min before the induction of ischemia. Serum and kidney tissue samples were subjected to biochemical and histopathological analyses.
Edaravone decreased the serum and tissue malondialdehyde levels in the ischemia/reperfusion group. The serum superoxide dismutase activity in the edaravone group was significantly higher than the ischemia/reperfusion and solvent groups. The serum nitric oxide level in the ischemia/reperfusion group was numerically higher than the sham group. The serum nitric oxide level was decreased by edaravone. The serum nitric oxide level was lower in the edaravone group than the solvent group. The tissue nitric oxide level was significantly higher in the ischemia/reperfusion than the sham group. In the ischemia/ reperfusion group, the histopathological changes were improved by edaravone.
Edaravone ameliorated renal injury caused by lower-limb ischemia/reperfusion. Therefore, it can be used to ameliorate acute ischemia/reperfusion injury during aortic and peripheral vascular surgery.
在本实验研究中，我们旨在探讨依达拉奉对大鼠急性下肢缺血/再灌注所致肾损伤的疗效。
2015年6月至2015年8月，本研究共使用40只雄性Wistar大鼠。将大鼠随机分为假手术组、缺血/再灌注组、依达拉奉组和溶剂组（每组10只）。夹闭肾下腹主动脉120分钟，然后在移除夹子后再灌注120分钟。在诱导缺血前30分钟静脉注射依达拉奉。对血清和肾组织样本进行生化和组织病理学分析。
依达拉奉降低了缺血/再灌注组的血清和组织丙二醛水平。依达拉奉组的血清超氧化物歧化酶活性显著高于缺血/再灌注组和溶剂组。缺血/再灌注组的血清一氧化氮水平在数值上高于假手术组。依达拉奉降低了血清一氧化氮水平。依达拉奉组的血清一氧化氮水平低于溶剂组。缺血/再灌注组的组织一氧化氮水平显著高于假手术组。在缺血/再灌注组中，依达拉奉改善了组织病理学变化。
依达拉奉减轻了下肢缺血/再灌注所致的肾损伤。因此，它可用于减轻主动脉和外周血管手术期间的急性缺血/再灌注损伤。
