Hsia J C, Wong L T, Deutsch H F
Biochim Biophys Acta. 1986 Feb 19;880(2-3):117-22. doi: 10.1016/0304-4165(86)90070-x.
The distribution of fatty acids and diethylstilbestrol between serum albumin and alpha-fetoprotein was measured in vitro by a new method based on the separation of the two proteins by virtue of the binding specificity of concanavalin A for the carbohydrate moiety of alpha-fetoprotein. Human and bovine proteins were investigated. It was found that palmitate and oleate were distributed almost equally between albumin and alpha-fetoprotein, while docosahexaenoate and diethylstilbestrol bound preferentially to alpha-fetoprotein even at an albumin: alpha-fetoprotein ratio of 10:1. The results confirm the binding specificity of alpha-fetoprotein for polyunsaturated fatty acids and also show that alpha-fetoprotein binds diethylstilbestrol much more strongly than albumin does. This suggests that alpha-fetoprotein may play a role in the fetal uptake of diethylstilbestrol.
通过一种基于伴刀豆球蛋白A对甲胎蛋白碳水化合物部分的结合特异性来分离这两种蛋白质的新方法，在体外测定了血清白蛋白和甲胎蛋白之间脂肪酸和己烯雌酚的分布情况。对人源和牛源蛋白质进行了研究。结果发现，棕榈酸酯和油酸酯在白蛋白和甲胎蛋白之间的分布几乎相等，而二十二碳六烯酸酯和己烯雌酚即使在白蛋白与甲胎蛋白的比例为10:1时也优先与甲胎蛋白结合。这些结果证实了甲胎蛋白对多不饱和脂肪酸的结合特异性，同时也表明甲胎蛋白结合己烯雌酚的能力比白蛋白强得多。这表明甲胎蛋白可能在胎儿摄取己烯雌酚过程中发挥作用。
