大鼠甲胎蛋白和白蛋白与多不饱和脂肪酸及其他脂肪酸的相互作用：表观缔合常数的测定

Interaction of rat alpha-fetoprotein and albumin with polyunsaturated and other fatty acids: determination of apparent association constants.

作者信息

Anel A, Calvo M, Naval J, Iturralde M, Alava M A, Piñeiro A

机构信息

Departamento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, Spain.

出版信息

FEBS Lett. 1989 Jun 19;250(1):22-4. doi: 10.1016/0014-5793(89)80676-3.

DOI:10.1016/0014-5793(89)80676-3

PMID:2472294

Abstract

The interaction of fatty acids with rat alpha-fetoprotein and albumin was measured using a partition equilibrium method. alpha-Fetoprotein (AFP) displays one high-affinity binding site for fatty acids and albumin near two binding sites. The AFP association constants for most fatty acids were similar to those of albumin (in the 10(7) M-1 range) whereas for docosahexaenoic acid it was 9.7 x 10(8) M-1, about 50-fold higher than that corresponding to albumin. This difference justifies docosahexaenoic acid in fetal or neonatal serum being mainly bound to AFP and can indicate a highly specific role of AFP in the transport of this fatty acid.

摘要

采用分配平衡法测定了脂肪酸与大鼠甲胎蛋白和白蛋白的相互作用。甲胎蛋白（AFP）对脂肪酸有一个高亲和力结合位点，而白蛋白有两个结合位点。大多数脂肪酸与AFP的结合常数与白蛋白的相似（在10⁷ M⁻¹范围内），而二十二碳六烯酸与AFP的结合常数为9.7×10⁸ M⁻¹，约为白蛋白相应结合常数的50倍。这种差异解释了胎儿或新生儿血清中的二十二碳六烯酸主要与AFP结合，并表明AFP在这种脂肪酸的转运中具有高度特异性作用。