从海参中发现的由新型四糖重复单元组成的岩藻聚糖结构解析。
Structure elucidation of fucoidan composed of a novel tetrafucose repeating unit from sea cucumber Thelenota ananas.
机构信息
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
出版信息
Food Chem. 2014 Mar 1;146:113-9. doi: 10.1016/j.foodchem.2013.09.033. Epub 2013 Sep 12.
Thelenota ananas is one of the most popular edible sea cucumber species consumed in China and some Southeast Asian countries. In this study, the delicate structure of fucoidan from T. ananas (Ta-FUC) was clarified. Oligosaccharides and low molecular weight polysaccharides of Ta-FUC were prepared by enzymatic degradation. Their structures, which retained primary structural features of Ta-FUC, were investigated using tandem mass spectrometry and nuclear magnetic resonance. As a result, Ta-FUC was found to be composed of a novel tetrafucose repeating unit [→3-α-L-Fucp-1→3-α-L-Fucp-1→3-α-L-Fucp2,4(OSO3(-))-1→3-α-L-Fucp2(OSO3(-))-1→]. Meanwhile, it was proven to possess a significant inhibitory effect on superoxide radicals with an IC50 of 17.46±0.14μg/ml. This effect was related to its sulphation pattern.
菠萝海地瓜是中国和一些东南亚国家食用的最受欢迎的海参品种之一。在这项研究中，澄清了菠萝海地瓜（Ta-FUC）岩藻聚糖的精细结构。通过酶解制备了 Ta-FUC 的寡糖和低分子量多糖。使用串联质谱和核磁共振研究了它们保留了 Ta-FUC 主要结构特征的结构。结果表明，Ta-FUC 由一个新型四糖重复单元[→3-α-L-Fucp-1→3-α-L-Fucp-1→3-α-L-Fucp2,4(OSO3(-))-1→3-α-L-Fucp2(OSO3(-))-1→]组成。同时，它被证明对超氧自由基具有显著的抑制作用，IC50 为 17.46±0.14μg/ml。这种作用与其硫酸化模式有关。
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