College of Food Science and Engineering , Ocean University of China , 5 Yushan Road , Qingdao 266003 , China.
College of Food Science and Light Industry , Nanjing Tech University , 30 South Puzhu Road , Nanjing 211816 , China.
J Agric Food Chem. 2018 Aug 15;66(32):8615-8622. doi: 10.1021/acs.jafc.8b01497. Epub 2018 Aug 6.
A variety of bioactivities has been established for fucoidan extracted from sea cucumber, whereas its structure-activity relationships have been seldom investigated. In this study, sea cucumber ( Thelenota ananas) fucoidans with different molecular weights were prepared with enzymatic degradation. The chain stiffness and molecular size decreased with the decreasing of molecular weight. Fucoidans with molecular weight of 1380.0, 828.7, and 483.0 kDa exhibited random coil conformations, while fucoidan molecular weight of 215.0 kDa existed as sphere in solution. All examined fucoidans could effectively prevent the ethanol-induced gastric ulcer, of which mechanism involved antioxidation and anti-inflammation. Within the range of the study, the performance of fucoidans adopted random coil conformations declined with the decreasing of molecular weight, and the performance recovered when the chain conformation transited from coil to sphere, indicating the subtle influences of molecular weight and chain conformation on the gastric protective activity of sea cucumber fucoidan.
从海参中提取的岩藻聚糖具有多种生物活性，但对其结构-活性关系的研究甚少。本研究采用酶解的方法制备了不同分子量的海参岩藻聚糖。随着分子量的降低，链的刚性和分子尺寸减小。分子量为 1380.0、828.7 和 483.0 kDa 的岩藻聚糖呈现无规卷曲构象，而分子量为 215.0 kDa 的岩藻聚糖在溶液中呈球形存在。所有检测到的岩藻聚糖都能有效预防乙醇诱导的胃溃疡，其作用机制涉及抗氧化和抗炎。在所研究的范围内，采用无规卷曲构象的岩藻聚糖的性能随着分子量的降低而下降，当链构象从卷曲过渡到球时，性能得到恢复，这表明分子量和链构象对海参岩藻聚糖胃保护活性的细微影响。
