碳化硅纤维介导的 DNA 递送至小麦（Triticum aestivum L.）成熟胚胎细胞中。

Silicon carbide fiber-mediated DNA delivery into cells of wheat (Triticum acstivum L.) mature embryos.

机构信息

Department of Botany, Faculty of Science, Kyoto University, 606-01, Sakyo-Ku, Kyoto, Japan.

出版信息

Plant Cell Rep. 1996 Dec;16(3-4):133-6. doi: 10.1007/BF01890853.

Abstract

We have demonstrated that foreign DNA can be delivered into cells of mature embryos of wheat (Triticum aestivum L.) using silicon carbide fibers (SCF). The highest transient expression of thegusA (GUS) gene was detected when dry embryos were vortexed for 10-30 min in a SCF-DNA solution containing 90-120 g/l of sucrose. Up to 100 (on average 20-40) blue expression units per embryo were observed. Scutellum side and epiblast of the intact wheat embryos are preferentially transformed. When embryos with the coleoptilar tip removed were treated and allowed to germinate, GUS staining was observed in emerging leaf tissues. The potential of this new approach for stable transformation of wheat is under investigation. It has been found that callus tissues induced from the SCF treated embryos contain GUS-expressing sectors one month after treatment.

摘要

我们已经证明，利用碳化硅纤维（SCF）可以将外源 DNA 递送到成熟小麦（Triticum aestivum L.）胚胎细胞中。当干燥的胚胎在含有 90-120 g/l 蔗糖的 SCF-DNA 溶液中涡旋 10-30 分钟时，检测到最高的 GusA（GUS）基因瞬时表达。每个胚胎观察到的蓝色表达单位多达 100（平均 20-40）个。优先转化完整小麦胚胎的盾片侧和外胚层。当去除了胚芽鞘顶端的胚胎进行处理并允许发芽时，在出现的叶片组织中观察到 GUS 染色。正在研究这种新方法对小麦稳定转化的潜力。已经发现，SCF 处理后的胚胎诱导的愈伤组织在处理后一个月含有表达 GUS 的组织。