Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, P.O.Box 521, H-6701, Szeged, Hungary.
Theor Appl Genet. 1994 Sep;89(1):1-8. doi: 10.1007/BF00226974.
A species-specific repetitive DNA fragment has been isolated from a genomic library of Solanum brevidens. Sequence analysis revealed a regular organization of three non-homologous subrepeats forming tandemly-arranged composite repetitive units. Interpretation of Southern hybridization patterns based on the known sequence data suggests that the isolated sequence element represents an abundant organization type, although the presence of simple tandem arrays of the subrepeats is also indicated. Seventy-four percent sequence similarity was found between one of the S. brevidens subrepeats (Sb4AX) and a satellite DNA (TGRI) localized as a subtelomeric repeat on almost all Lycopersicon esculentum chromosomes. Insitu hybridization indicated that, similarly to TGRI, the S. brevidens-specific repeats are located at the ends of the arms of several chromosomes. On the basis of the data obtained, a common ancestral sequence can be proposed for the tomato (TGRI) and the S. brevidens (Sb4AX) repeat however, the molecular organization of this element in these two species evolved in a basically different manner.
已从 Solanum brevidens 的基因组文库中分离出一种种特异性重复 DNA 片段。序列分析显示，三个非同源亚重复序列以串联排列的方式形成规则的组织形式，形成复合重复单元。根据已知序列数据对 Southern 杂交模式的解释表明，尽管存在简单的亚重复串联排列，但分离出的序列元件代表一种丰富的组织类型。在 Lycopersicon esculentum 染色体的几乎所有端粒上都发现了一种称为 TGRI 的卫星 DNA，其与 S. brevidens 的一个亚重复序列（Sb4AX）之间存在 74%的序列相似性。原位杂交表明，与 TGRI 相似，S. brevidens 特异性重复序列位于几个染色体臂的末端。根据获得的数据，可以为番茄（TGRI）和 S. brevidens（Sb4AX）重复序列提出一个共同的祖先序列，然而，这两个物种中该元件的分子组织以基本不同的方式进化。
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