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蛔虫肠道:氧气消耗、发酵酸及蛋白质的厌氧合成

Intestine of Ascaris: oxygen consumption, fermentation acids, and anaerobic synthesis of protein.

作者信息

Harpur R P, Jackson D M

出版信息

J Parasitol. 1975 Oct;61(5):808-14.

PMID:241780
Abstract

The oxygen uptakes by pieces of gut from the porcine roundworm, Ascaris suum, under Po2 10 to 710 torr were from 0.6 to 3.0 mul/mg dry wt/hr (Qo2). Increasing concentrations of tissue, 30 to 90 mg (dry wt/3 ml) decreased Qo2 in air from 1.9 to 1.1 and only technical grade catalase was found to relieve this inhibition. Conversely aerobic fermentation, judged by acid production, was directly proportional to the amount of tissue present: the acids from this fermentation were 2-methyl-butyric (1% of the total), succinic (8%), propionic (40%), and acetic (51%). Glucose did not increase Qo2 but it doubled the incorporation of labeled carbon into protein from glycine-1-14C. Judged by this, protein synthesis proceeded at the same rate under low Po2 (0.01 torr) as under air.

摘要

在氧分压为10至710托的条件下,猪蛔虫(Ascaris suum)肠道组织的耗氧量为0.6至3.0微升/毫克干重/小时(呼吸商)。组织浓度从30毫克增加到90毫克(干重/3毫升),会使空气中的呼吸商从1.9降至1.1,并且发现只有工业级过氧化氢酶能缓解这种抑制作用。相反,通过产酸判断的需氧发酵与组织量成正比:这种发酵产生的酸为2-甲基丁酸(占总量的1%)、琥珀酸(8%)、丙酸(40%)和乙酸(51%)。葡萄糖不会增加呼吸商,但会使甘氨酸-1-¹⁴C中标记碳掺入蛋白质的量增加一倍。据此判断,在低氧分压(0.01托)下蛋白质合成的速率与在空气中相同。

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