Japan Turfgrass Inc., 3-6-2 Akanehama, Narashino-shi, 275, Chiba, Japan.
Plant Cell Rep. 1996 Jun;15(10):737-41. doi: 10.1007/BF00232218.
Embryogenic callus of Japanese lawngrass (Zoysia japonica Steud.) was induced from sterile mature seeds on LS medium with 5 mg / l of 2,4-D. Embryogenic callus selected visually under microscope was proliferated in liquid N6 medium with amino acids (N6-AA medium). Protoplasts were isolated from suspension cells by the treatment of enzyme mixture containing pectolyase Y-23 and cultured in K8p medium with 2 mg / l of 2,4-D at the density of 10(6) / ml. Plants were regenerated by transferring the protoplasts derived callus to MS medium and incubating at 28 °C under light for two months. Plantlets were successfully transplanted in the soil.
日本结缕草（Zoysia japonica Steud.）的胚性愈伤组织从无菌成熟种子在 LS 培养基上诱导，培养基中添加 5mg/L 的 2,4-D。在显微镜下选择胚性愈伤组织，在含氨基酸的液体 N6 培养基（N6-AA 培养基）中增殖。原生质体通过含有果胶酶 Y-23 的酶混合物处理从悬浮细胞中分离出来，并在 K8p 培养基中，以 2mg/L 的 2,4-D 在密度为 10(6)/ml 的条件下培养。通过将衍生愈伤组织的原生质体转移到 MS 培养基中，并在 28°C 光照下孵育两个月，来再生植株。植株成功地移栽到土壤中。
