Departments of Crop and Soil Sciences and of Entomology, 202 Pesticide Research Center, Michigan State University, 48824-1311, East Lansing, MI, USA.
Plant Cell Rep. 1993 Nov;13(1):1-6. doi: 10.1007/BF00232305.
Transgenic creeping bentgrass (Agrostis palustris Huds., cv. Pencross; Poaceae) plants have been obtained by microprojectile bombardment of and regeneration from embryogenic calli with a vector designed to deliver the β-glucuronidase (GUS) gene under the control of rice actin 1 5' regulatory sequences. Southern analysis of polymerase chain reaction (PCR)-amplified and restriction-digested genomic DNA of four transgenic plants regenerated from these cultures showed the unscrambled integration of the gus fragment. Northern blot analysis confirmed the expression of gus mRNA in one of the transgenic plants. Western blot analysis revealed a high level of accumulation of gus protein. Histochemical assays showed enzymatic activity of β-glucuronidase in all parts of the transgenic turfgrass plant. The order of gus expression level in different tissues of the transgenic plant is as follows: stem node > first young leaf > root tip > second / third / fourth young leaf > stem internode > root hair-zone.
通过微弹丸轰击和从胚性愈伤组织再生，获得了转基因匍匐翦股颖（Agrostis palustris Huds.，cv. Pencross；禾本科）植物。该载体旨在将β-葡萄糖醛酸酶（GUS）基因在水稻肌动蛋白 1 5'调控序列的控制下传递。对从这些培养物再生的四个转基因植物的聚合酶链反应（PCR）扩增和限制性酶切基因组 DNA 的Southern 分析表明，gus 片段的未重排整合。Northern blot 分析证实了其中一个转基因植物中 gus mRNA 的表达。Western blot 分析显示 Gus 蛋白的高水平积累。组织化学分析显示转基因草坪植物的所有部位均具有β-葡萄糖醛酸酶的酶活性。转基因植物不同组织中 gus 表达水平的顺序如下：茎节＞第一幼叶＞根尖＞第二/第三/第四幼叶＞茎节间＞根毛区。
