大规模筛查人血清中的 HCV RNA 和 GBV-C RNA。
Large scale screening of human sera for HCV RNA and GBV-C RNA.
机构信息
University of North Carolina, Chapel Hill, North Carolina.
出版信息
J Med Virol. 2014 Mar;86(3):473-7. doi: 10.1002/jmv.23829. Epub 2013 Oct 31.
Abstract
North Carolina locates acute HIV cases by pooled nucleic acid testing of HIV-antibody negative serum samples. Here, 224 pools of 80 HIV-negative samples (N = 17,920) were screened for viral RNA from HCV, GBV-C, and influenza A. No evidence of influenza A was found, but HCV and GBV-C were common (1.2% and 1.7% prevalence, respectively), demonstrating the utility of pooled testing in locating individuals that may remain undiagnosed otherwise. By sequencing positive pools, potential transmission clusters may be located as well.
摘要
北卡罗来纳州通过对 HIV 抗体阴性血清样本进行聚合酶链反应（pooled nucleic acid testing，简称 PNA）来定位急性 HIV 感染病例。在此，对 224 组 80 份 HIV 阴性样本（N=17920）进行了丙型肝炎病毒（hepatitis C virus，简称 HCV）、庚型肝炎病毒（hepatitis G virus，简称 GBV-C）和甲型流感病毒（influenza A virus，简称 IAV）的 RNA 检测。结果未发现甲型流感病毒，但 HCV 和 GBV-C 较为常见（分别为 1.2%和 1.7%的流行率），这表明聚合检测在定位可能未被诊断的个体方面具有实用性。通过对阳性样本进行测序，还可以定位潜在的传播集群。
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