Sun Linlin, Li Dongni, Hemraz Usha D, Fenniri Hicham, Webster Thomas J
Bioengineering Program and Department of Chemical Engineering, College of Engineering, Northeastern University, Boston, Massachusetts, 02115.
J Biomed Mater Res A. 2014 Oct;102(10):3446-51. doi: 10.1002/jbm.a.35008. Epub 2013 Nov 1.
The next generation skin of wound healing materials should stimulate skin regeneration by actively promoting appropriate cellular adhesion and proliferation. As materials with novel self-assembling and solidification properties when transitioning from room to body temperatures, rosette nanotubes (RNTs) may be such a proactive material. RNTs resemble naturally occurring nanostructures in the skin (such as collagen and keratin) assembling with noncovalent forces in physiological environments. Presenting desirable bioactive properties, RNTs have been used for various tissue engineering applications including increasing in vivo bone and cartilage regeneration. The objective of the current in vitro study was, for the first time, to improve properties of a commonly used hydrogel (poly(2-hydroxyethyl methacrylate) or pHEMA) for skin regeneration by incorporating one type of novel self-assembled RNTs, called TBL. Results showed for the first time increased keratinocyte and fibroblast proliferation on hydrogels coated with TBLs compared to those not coated with TBL. In this manner, this study provides the first evidence that TBL RNTs are promising for wound healing applications due to their optimal cytocompatibility, solidification, and mechanical properties and, thus, should be further studied for such applications.
下一代伤口愈合材料应通过积极促进适当的细胞黏附和增殖来刺激皮肤再生。作为从室温转变为体温时具有新型自组装和固化特性的材料，玫瑰花结纳米管（RNTs）可能就是这样一种具有前瞻性的材料。RNTs类似于皮肤中天然存在的纳米结构（如胶原蛋白和角蛋白），在生理环境中通过非共价力组装而成。由于具有理想的生物活性特性，RNTs已被用于各种组织工程应用，包括促进体内骨骼和软骨再生。当前这项体外研究的目的首次是通过掺入一种名为TBL的新型自组装RNTs来改善常用水凝胶（聚甲基丙烯酸2-羟乙酯或pHEMA）用于皮肤再生的性能。结果首次表明，与未涂覆TBL的水凝胶相比，涂覆TBL的水凝胶上角质形成细胞和成纤维细胞的增殖增加。通过这种方式，本研究首次提供了证据，表明TBL RNTs因其最佳的细胞相容性、固化和机械性能而有望用于伤口愈合应用，因此，应进一步研究其在这类应用中的作用。
