Cho Jae-Young, Bhowmik Pankaj, Polowick Patricia L, Dodard Sabine G, El-Bakkari Mounir, Nowak Goska, Fenniri Hicham, Hemraz Usha D
Nanotechnology Research Centre, National Research Council of Canada, 11421 Saskatchewan Drive, Edmonton, Alberta T6G 2M9, Canada.
Aquatic and Crop Resource Development, National Research Council of Canada, 110 Gymnasium Place, Saskatoon, Saskatchewan S7N 0W9, Canada.
ACS Omega. 2020 Sep 16;5(38):24422-24433. doi: 10.1021/acsomega.0c02830. eCollection 2020 Sep 29.
Plant genetic engineering offers promising solutions to the increasing demand for efficient, sustainable, and high-yielding crop production as well as changing environmental conditions. The main challenge for gene delivery in plants is the presence of a cell wall that limits the transportation of genes within the cells. Microspores are plant cells that are, under the right conditions, capable of generating embryos, leading to the formation of haploid plants. Here, we designed cationic and fluorescent rosette nanotubes (RNTs) that penetrate the cell walls of viable wheat microspores under mild conditions and in the absence of an external force. These nanomaterials can capture plasmid DNA to form RNT-DNA complexes and transport their DNA cargo into live microspores. The nanomaterials and the complexes formed were nontoxic to the microspores.
植物基因工程为满足对高效、可持续和高产作物生产不断增长的需求以及应对不断变化的环境条件提供了有前景的解决方案。植物基因传递面临的主要挑战是存在细胞壁，这限制了基因在细胞内的运输。小孢子是植物细胞，在合适的条件下能够产生胚胎，进而形成单倍体植物。在此，我们设计了阳离子荧光玫瑰花结纳米管（RNTs），其能够在温和条件下且无需外力的情况下穿透活小麦小孢子的细胞壁。这些纳米材料可以捕获质粒DNA形成RNT-DNA复合物，并将其DNA负载转运到活的小孢子中。所形成的纳米材料和复合物对小孢子无毒。
