Robenek H, Schmitz G
Eur J Cell Biol. 1985 Nov;39(1):77-85.
Using the platinum-carbon surface replication technique, the distribution of receptors for two colloidal gold labeled lipoproteins (acetylated low density lipoprotein and high density lipoprotein), iron-saturated transferrin and bovine serum albumin in the plasma membrane of cultured mouse peritoneal macrophages was mapped. The plasma membrane surface of cultured peritoneal macrophages exhibits three morphologically distinct regions which have shown differences in the distribution, density and dynamics of these receptors. The flat border of the plasma membrane surfaces are the domains that appear to have the highest concentration of transferrin receptors, whereas the intermediate regions contain most of the receptors for acetylated low density lipoprotein and high density lipoprotein. Bovine serum albumin receptors are concentrated at the edge of the cells and had a uniform distribution on the rest of the cell surface. The functional significance and the mechanisms by which these regional differences in the distribution of the receptors are generated and then maintained are not yet known.
采用铂-碳表面复制技术，绘制了培养的小鼠腹腔巨噬细胞质膜中两种胶体金标记脂蛋白（乙酰化低密度脂蛋白和高密度脂蛋白）、铁饱和转铁蛋白和牛血清白蛋白受体的分布图谱。培养的腹腔巨噬细胞质膜表面呈现出三个形态上不同的区域，这些区域在这些受体的分布、密度和动态方面存在差异。质膜表面的平坦边界区域似乎具有最高浓度的转铁蛋白受体，而中间区域则含有大部分乙酰化低密度脂蛋白和高密度脂蛋白的受体。牛血清白蛋白受体集中在细胞边缘，在细胞表面的其余部分分布均匀。这些受体分布区域差异产生并维持的功能意义和机制尚不清楚。
