Robenek H, Schmitz G, Assmann G
J Histochem Cytochem. 1984 Oct;32(10):1017-27. doi: 10.1177/32.10.6481148.
The topography and dynamics of receptors for acetylated (acetyl) and malondialdehyde-modified (MDA) low-density lipoprotein (LDL) in the plasma membrane of cultured mouse peritoneal macrophages were investigated using a new technique. Modified LDL labeled with gold particles was used to visualize LDL receptors in the plane of the plasma membrane in platinum-carbon surface replicas of critical point-dried cells. It was found that the native distribution of unoccupied acetyl-LDL receptors is diffuse, whereas unoccupied MDA-LDL receptors are preclustered in the plasma membrane. Competition and double labeling experiments suggest the existence of two distinct classes of receptor sites for acetyl-LDL and MDA-LDL.
运用一项新技术，研究了培养的小鼠腹膜巨噬细胞质膜中乙酰化（乙酰基）和丙二醛修饰（MDA）的低密度脂蛋白（LDL）受体的拓扑结构和动力学。用金颗粒标记的修饰LDL用于在临界点干燥细胞的铂 - 碳表面复制品中可视化质膜平面中的LDL受体。发现未占据的乙酰 - LDL受体的天然分布是弥散的，而未占据的MDA - LDL受体在质膜中预先聚集。竞争和双重标记实验表明存在两类不同的乙酰 - LDL和MDA - LDL受体位点。
