Department of Medical Protein Research, VIB, B-9000, Ghent, Belgium; Department of Biochemistry, Ghent University, B-9000, Ghent, Belgium.
Mass Spectrom Rev. 2014 Mar-Apr;33(2):147-56. doi: 10.1002/mas.21386. Epub 2013 Oct 31.
The oxidation and consequent reduction of protein-bound methionine residues is of great interest in understanding different aspects of how oxidative stress affects protein functions and cellular signaling. To date, few technologies are available for the study of methionine sulfoxides. And, especially the absence of highly specific antibodies has impeded the field in understanding the exact role of methionine oxidation on a proteome-wide level. Nonetheless, the different models where the responsible enzymes for repair of the oxidized methionines have been studied show that there is an important role for this modification in a cellular context. We here review different mass spectrometry based and proteomics methods for characterizing in vivo methionine oxidation.
蛋白质结合的蛋氨酸残基的氧化和随之而来的还原在理解氧化应激如何影响蛋白质功能和细胞信号方面非常重要。迄今为止，用于研究蛋氨酸亚砜的技术很少。而且，特别是缺乏高度特异性的抗体，阻碍了该领域在全蛋白质组水平上了解蛋氨酸氧化的确切作用。尽管如此，研究负责修复氧化蛋氨酸的酶的不同模型表明，这种修饰在细胞环境中具有重要作用。在这里，我们综述了基于质谱的不同方法和蛋白质组学方法，用于研究体内蛋氨酸氧化。
