Centro de Investigaciones de Plagas e Insecticidas (CIPEIN/ UNIDEF-CONICET), J.B. de La Salle 4397, (1603) Villa Martelli, Buenos Aires, Argentina.
J Med Entomol. 2013 Sep;50(5):1046-54. doi: 10.1603/me12248.
The effect on locomotor activity, the repellency, and the knock-down produced by 10 monoterpene alcohols were evaluated on first-instar nymphs of Rhodnius prolixus and Triatoma infestans, vectors of Chagas disease. A video tracking technique was used to evaluate locomotor activity and repellency by exposure to papers impregnated with monoterpenes. Eugenol on R. prolixus and (S)-cis-verbenol on T. infestans did not modify the locomotor activity. The remaining monoterpenes produced hyperactivity on both species, although the concentration required was at least a 1,000 times higher than that of deltamethrin (positive control). Carvacrol, eugenol, and geraniol resulted as repellent as N,N-diethyl-m-toluamide (positive control) for both species. A similar result was observed for almost every monoterpene on T. infestans. Knock-down effect was evaluated by exposing the nymphs in closed recipients. The order of increasing toxicity on R. prolixus was (KT50 values in min): geraniol (213.7) < alpha-terpineol (164.5) < linalool (124.2) < carvacrol (111.6) < eugenol (89.8) < thymol (78.9), and on T. infestans: alpha-terpineol (289.8) < eugenol (221.3) < carvacrol (164.2) < linalool (154.9) < thymol (96.7). All monoterpenes were less toxic than the positive control, dichlorvos (3.6 min for R. prolixus and 3.9 min for T. infestans). After 7 h of exposure, (-)-carveol, citronellol, and menthol (on both species) and geraniol (on T. infestans) produced < 50% of knock-down. After these results, it is worthwhile to explore more deeply the potential of these compounds as tools for controlling Chagas disease vectors.
评估了 10 种单萜醇对感染恰加斯病的传播媒介 Rhodnius prolixus 和 Triatoma infestans 一龄若虫的运动活性、驱避性和击倒作用。通过暴露于浸渍单萜的纸张，使用视频跟踪技术来评估运动活性和驱避性。丁香酚对 R. prolixus 和（S）-顺式马鞭草烯醇对 T. infestans 没有改变运动活性。其余的单萜醇对这两种物种都产生了过度活跃的作用，尽管所需的浓度至少比溴氰菊酯（阳性对照）高 1000 倍。香芹酚、丁香酚和香叶醇对这两种物种的驱避效果与 N，N-二乙基间甲苯酰胺（阳性对照）一样好。几乎每一种单萜醇对 T. infestans 都有类似的结果。通过将若虫暴露在封闭的容器中评估击倒效果。对 R. prolixus 的毒性增加顺序为（KT50 值，分钟）：香叶醇（213.7）<α-松油醇（164.5）<芳樟醇（124.2）<香芹酚（111.6）<丁香酚（89.8）<百里酚（78.9），对 T. infestans 为：α-松油醇（289.8）<丁香酚（221.3）<香芹酚（164.2）<芳樟醇（154.9）<百里酚（96.7）。所有的单萜醇都比阳性对照二氯氧磷毒性小（R. prolixus 为 3.6 分钟，T. infestans 为 3.9 分钟）。暴露 7 小时后，(-)-香芹醇、香茅醇和薄荷醇（对两种物种）和香叶醇（对 T. infestans）产生的击倒率<50%。根据这些结果，值得更深入地探索这些化合物作为控制恰加斯病传播媒介的工具的潜力。
