钙离子载体A23187可诱发并增强干扰素的抗病毒活性。

The calcium ionophore A23187 evokes and potentiates antiviral activity of interferon.

作者信息

Maheshwari R K, Husain M M, Friedman R M, Krishna G

出版信息

J Interferon Res. 1985 Fall;5(4):605-12. doi: 10.1089/jir.1985.5.605.

DOI:10.1089/jir.1985.5.605

Abstract

Calcium ionophore A23187, which causes a rapid efflux of Ca2+ from cells, evokes an antiviral response in mouse LB, simian COS-1, Hela, human amniotic (U), baby hamster kidney (BHK), and VERO cells against Sindbis (SBV) and vesicular stomatitis (VSV) viruses. The degree of antiviral activity depends on the type of cell, virus, and the dose of A23187. A23187 inhibits the production of infectious VSV; however, VSV particle production was not significantly inhibited as measured by viral RNA and viral proteins. The VSV released from the A23187-treated cells is deficient in VSV glycoprotein (G) and membrane (M) protein. A23187 potentiates the antiviral activity of interferon (IFN) against SBV and VSV in mouse LB and human U cells. It is possible to postulate that a change in intracellular Ca2+ may play an important role in the antiviral activity of IFN.

摘要

钙离子载体A23187可使细胞内的Ca2+快速外流，它能在小鼠LB细胞、猴COS-1细胞、Hela细胞、人羊膜（U）细胞、幼仓鼠肾（BHK）细胞和VERO细胞中引发针对辛德毕斯病毒（SBV）和水疱性口炎病毒（VSV）的抗病毒反应。抗病毒活性的程度取决于细胞类型、病毒以及A23187的剂量。A23187可抑制传染性VSV的产生；然而，通过病毒RNA和病毒蛋白检测发现，VSV颗粒的产生并未受到显著抑制。从经A23187处理的细胞中释放出的VSV缺乏VSV糖蛋白（G）和膜蛋白（M）。在小鼠LB细胞和人U细胞中，A23187可增强干扰素（IFN）对SBV和VSV的抗病毒活性。可以推测，细胞内Ca2+的变化可能在IFN的抗病毒活性中发挥重要作用。