Hightower M, Gross G W
Stain Technol. 1985 Nov;60(6):315-20. doi: 10.3109/10520298509113930.
Bodian and Nissl procedures were combined to stain dissociated mouse spinal cord cells cultured on coverslips. The Bodian technique stains fine neuronal processes in great detail as well as an intracellular fibrillar network concentrated around the nucleus and in proximal neurites. The Nissl stain clearly delimits neuronal cytoplasm in somata and in large dendrites. A combination of these techniques allows the simultaneous depiction of neuronal perikarya and all afferent and efferent processes. Costaining with little background staining by either procedure suggests high specificity for neurons. This procedure could be exploited for routine network analysis of cultured neurons.
将博迪安氏法和尼氏染色法结合起来，对培养在盖玻片上的解离小鼠脊髓细胞进行染色。博迪安氏技术能非常详细地染出精细的神经突，以及集中在细胞核周围和近端神经突中的细胞内纤维状网络。尼氏染色能清晰界定胞体和大型树突中的神经元细胞质。这两种技术的结合能同时描绘出神经元胞体以及所有传入和传出神经突。两种方法的共染色背景染色少，表明对神经元具有高特异性。该方法可用于培养神经元的常规网络分析。
