Cowie R J, Skalko R G, Baisden R H
Stain Technol. 1980 May;55(3):177-81. doi: 10.3109/10520298009067235.
A simple method for staining nerve cells and fibers of the salamander central nervous system is described. The procedure employs Carnoy's fixation followed by Protargol inpregnation and Nissl staining. This technique permits the simultaneous observation of intracellular neurofibrils, neuronal processes and basophilic components of the neuron. In addition, it eliminates the need to stain alternate sections with separate procedures to view the various components of the urodele central nervous system.
本文描述了一种用于染色蝾螈中枢神经系统神经细胞和纤维的简单方法。该程序采用卡诺固定法，随后进行原银浸染和尼氏染色。这种技术允许同时观察细胞内神经原纤维、神经元突起和神经元的嗜碱性成分。此外，它无需用单独的程序对交替切片进行染色，就能观察有尾目动物中枢神经系统的各种成分。
