Department of Biology, University of Southampton, Bassett Crescent East, S016 7PX, Southampton, United Kingdom.
Microb Ecol. 1995 Sep;30(2):157-70. doi: 10.1007/BF00172571.
A procedure has been developed for preparing living bacteria, quantitatively labeled with (3)H-thymidine and (14)C-leucine, for short-term grazing experiments. The negligible rate of accumulation in protozoan macromolecules of moieties of bacterial macromolecules labeled with (3)H compared with moieties labeled with (14)C permits estimation of the consumption, digestion, and assimilation of prey biomass in protists without separating them from bacteria. The principles of this method are described, and the results of its application in examples of grazing by the ciliates Euplotes and Uronema and the flagellate Pteridomonas on the bacterium Vibrio are outlined.
已经开发出一种程序，用于制备用（3）H-胸腺嘧啶和（14）C-亮氨酸进行定量标记的活体细菌，用于短期放牧实验。与用（14）C 标记的细菌大分子的部分相比，用（3）H 标记的原生动物大分子的部分在原生动物中的积累率可忽略不计，这允许在不将它们与细菌分离的情况下估计原生动物中猎物生物量的消耗、消化和同化。本文介绍了该方法的原理，并概述了该方法在纤毛虫 Euplotes 和 Uronema 以及鞭毛藻 Pteridomonas 对细菌 Vibrio 进行放牧的实例中的应用结果。
