INRA, C.R. d'Angers, Station d'Amélioration des Espèces Fruitières et Ornementales, F-49071, Beaucouzé, France.
Plant Cell Rep. 1995 Jan;15(1-2):63-7. doi: 10.1007/BF01690255.
TransgenicPelargonium X hortorum have been producedvia Agrobacterium tumefaciens-mediated transformation. The regeneration protocol used provided a regeneration frequency approximately to 95 percent. Clumps of regenerants, from cotyledons and hypocotyls ofPelargonium X hortorum seedlings, were inoculated with the disarmed strain EHA101 ofAgrobacterium tumefaciens. This strain contains a binary vector carrying neomycin phosphotransferase II, hygromycin B phosphotransferase and ß-glucuronidase genes. Selection on the regeneration medium supplemented with hygromycin allowed production of transgenic plants in up to 20% of the inoculated explants. The insertion of foreign DNA was demonstrated by Southern and polymerase chain reaction analysis: these experiments indicated that the inserted T-DNA is not full length for most of the plants. All RO transgenic plants exhibited a normal phenotype and are fertile.
通过根癌农杆菌介导的转化方法已成功获得Pelargonium X hortorum 的转基因植株。所使用的再生方案可使再生频率达到约 95%。将丛生芽从 Pelargonium X hortorum 幼苗的子叶和下胚轴接种到根癌农杆菌的减毒菌株 EHA101 中。该菌株含有一个携带新霉素磷酸转移酶 II、潮霉素 B 磷酸转移酶和β-葡萄糖醛酸酶基因的二元载体。在添加潮霉素的再生培养基上进行选择，可使多达 20%的接种外植体产生转基因植株。通过 Southern 和聚合酶链反应分析证明了外源 DNA 的插入：这些实验表明，对于大多数植物来说，插入的 T-DNA 不是全长。所有 RO 转基因植株均表现出正常的表型且可育。
