Department of Horticultural Science, University of Guelph, N1G 2W1, Guelph, Ontario, Canada.
Plant Cell Rep. 1992 Aug;11(9):443-8. doi: 10.1007/BF00232687.
Intact seedlings of hybrid seed geranium (Pelargonium x hortorum Bailey) were tested for their ability to produce adventitious shoots and somatic embryos by direct culture of mature seeds on Murashige and Skoog (1962) medium (MS) supplemented with growth regulators BAP, BAP + IAA, or thidiazuron (TDZ). Ten varieties were tested in the presence of different BAP concentrations, four with BAP + IAA, and two with TDZ. Varieties used in this study differed in their response to BAP in the medium. Multiple adventitious shoots were produced by seven of the ten varieties tested. Multiple adventitious shoots were induced at all levels of TDZ in the medium. TDZ also induced callusing from roots and direct embryogenesis from intact hypocotyls. Adventitious shoots were separated, rooted and transferred to soil where they grew as normal healthy plants and flowered.
杂种天竺葵（Pelargonium x hortorum Bailey）完整的幼苗被测试是否能够通过在补充有生长调节剂 BAP、BAP+IAA 或噻苯隆（TDZ）的 Murashige 和 Skoog（1962）培养基（MS）上直接培养成熟种子来产生不定芽和体细胞胚。十种品种在不同 BAP 浓度下进行了测试，其中四种使用 BAP+IAA，两种使用 TDZ。本研究中使用的品种对培养基中的 BAP 反应不同。十种测试品种中有七种产生了多个不定芽。在培养基中所有 TDZ 水平都诱导了不定芽。TDZ 还可以诱导根的愈伤组织和完整下胚轴的直接胚胎发生。不定芽被分离、生根并转移到土壤中，在那里它们像正常健康的植物一样生长并开花。
