高效的籼稻（第 1 组）原生质体植株再生，来自一个优良品系和三个品种。
Efficient plant regeneration from Indica (group 1) rice protoplasts of one advanced breeding line and three varieties.
机构信息
Tissue Culture Laboratory, The International Rice Research Institute, P.O. Box 933, 1099, Manila, Philippines.
出版信息
Plant Cell Rep. 1995 Jan;15(1-2):68-71. doi: 10.1007/BF01690256.
Regenerable embryogenic suspensions were established from one Indica (group 1) rice advanced breeding line and 9 Indica (group 1) rice varieties in 6-8 weeks. Four were chosen for protoplast culture and plant regeneration. About 4-7×10(7) protoplasts were isolated from one gram of 8-week-old cell suspension. High plating efficiency (30.5%) and colony formation (13.7%) were obtained using nurse culture methods. A high plant regeneration frequency (67.5%) was observed for line IR57311-95-2-3. In total, 322 plants were regenerated. All the regenerated plants were fertile.
可再生胚胎悬浮系于 6-8 周内从一个籼稻（第 1 组）水稻先进育种系和 9 个籼稻（第 1 组）水稻品种中建立。其中 4 个品种用于原生质体培养和植株再生。从 8 周龄细胞悬浮系的 1 克中分离出约 4-7×10(7)个原生质体。采用看护培养法获得了高的平板效率（30.5%）和集落形成率（13.7%）。IR57311-95-2-3 系的植株再生频率很高（67.5%）。总共再生了 322 株植物。所有再生的植物都是可育的。
Zotero 插件