Tissue Culture Laboratory, The International Rice Research Institute, P.O. Box 933, 1099, Manila, Philippines.
Plant Cell Rep. 1995 Jan;15(1-2):68-71. doi: 10.1007/BF01690256.
Regenerable embryogenic suspensions were established from one Indica (group 1) rice advanced breeding line and 9 Indica (group 1) rice varieties in 6-8 weeks. Four were chosen for protoplast culture and plant regeneration. About 4-7×10(7) protoplasts were isolated from one gram of 8-week-old cell suspension. High plating efficiency (30.5%) and colony formation (13.7%) were obtained using nurse culture methods. A high plant regeneration frequency (67.5%) was observed for line IR57311-95-2-3. In total, 322 plants were regenerated. All the regenerated plants were fertile.
可再生胚胎悬浮系于 6-8 周内从一个籼稻（第 1 组）水稻先进育种系和 9 个籼稻（第 1 组）水稻品种中建立。其中 4 个品种用于原生质体培养和植株再生。从 8 周龄细胞悬浮系的 1 克中分离出约 4-7×10(7)个原生质体。采用看护培养法获得了高的平板效率（30.5%）和集落形成率（13.7%）。IR57311-95-2-3 系的植株再生频率很高（67.5%）。总共再生了 322 株植物。所有再生的植物都是可育的。
