Swiss Federal Institute of Technology, ETH-Zürich, Institute of Plant Sciences, CH-8092, Zürich, Switzerland.
Plant Cell Rep. 1990 Sep;9(5):253-6. doi: 10.1007/BF00232295.
Rice plants (Oryza sativa L., Chinsurah Boro II var. Indica) were regenerated from protoplasts isolated from microspore derived cell suspensions. A simple procedure for the establishment of such cell suspension cultures from embryogenic microcallus derived from cultured isolated microspores of Indica-type rice is described. Regenerating protoplasts could readily be isolated from 5-12 months old cell suspensions showing visible colony formation in the range of 180-1050 colonies/10(6) protoplasts after about one month in culture. More than 100 independent green plantlets were regenerated via secondary embryogenesis from ca 20×10(6) protoplasts. Out of 32 plants grown to maturity under greenhouse conditions 24 were fertile.
籼稻（Oryza sativa L.，Chinsurah Boro II var.Indica）可由原生质体再生而来，这些原生质体是从小孢子衍生细胞悬浮液中分离得到的。本文描述了一种简单的方法，可从胚性愈伤组织诱导的微孢子培养物中建立此类细胞悬浮培养物，该方法来源于籼稻类型的水稻。在培养约一个月后，在可见集落形成的范围内（180-1050 个集落/106 个原生质体），可以从 5-12 个月龄的细胞悬浮液中轻松分离出再生原生质体。通过二次胚胎发生，从约 20×106 个原生质体中再生了 100 多个独立的绿色小植株。在温室条件下生长至成熟的 32 株植物中，有 24 株具有育性。
