Plant Tissue and Cell Culture Facility, Post-Graduate Department of Botany, Utkal University, Bhubaneswar, -, 751004, Orissa, India.
Plant Cell Rep. 1996 Aug;15(11):841-5. doi: 10.1007/BF00233153.
High frequency bud break and multiple shoots were induced in nodal explants collected between November to February from a 5 year old tree of Morus australis Poir syn. M. acidosa Griff. on Murashige and Skoog's medium supplemented with 6-benzylaminopurine (1.0 mg/1). Incorporation of gibberellic acid (0.3 mg/l) along with BAP (1.0 mg/l) not only induced faster bud break from nodal explants as well as from apical shoot buds, but it also enhanced the frequency of bud break. Nodal explants were more responsive than apical shoot buds. The shoots formed in vitro were multiplied further as nodal segments, and an average multiplication rate of 6-fold per subculture was established within 4-5 months. The shoots were successfully rooted on half-strength MS containing a combination of indole-3-acetic acid, indole-3-butyric acid and indole-3-propionic acid, each at 1.0 mg/1. The plantlets were successfully hardened off and established in natural soil.
在 11 月至 2 月间，从一棵 5 年生的毛叶桑（Morus australis Poir syn. M. acidosa Griff.）树上采集的节间外植体，在添加 6-苄基氨基嘌呤（1.0 mg/1）的 Murashige 和 Skoog 培养基上，高频芽断裂和多次芽诱导。加入赤霉素（0.3 mg/l）和 BAP（1.0 mg/l）不仅能更快地从节间外植体和顶芽芽中诱导芽断裂，还能提高芽断裂的频率。节间外植体比顶芽芽更有反应性。在体外形成的芽进一步作为节段进行增殖，在 4-5 个月内建立了平均每继代 6 倍的增殖率。将芽成功地在含有吲哚-3-乙酸、吲哚-3-丁酸和吲哚-3-丙酸各 1.0 mg/1 的半强度 MS 上生根。将植物成功地硬化并在自然土壤中建立。
