通过组织培养克隆印度沙漠中的 Maytenus emarginata（Willd.）Ding Hou 树。

Cloning of Maytenus emarginata (Willd.) Ding Hou - a tree of the Indian Desert, through tissue culture.

机构信息

Plant Biotechnology Laboratory, Department of Botany, University of Jodhpur, 342001, Jodhpur, India.

出版信息

Plant Cell Rep. 1992 Aug;11(9):449-51. doi: 10.1007/BF00232688.

PMID:24201587

Abstract

An in vitro method for cloning and mass multiplication of Maytenus emarginata, a highly drought resistant tree of the Indian Desert, has been developed. Shoot segments harvested from a "plus" tree (30-year-old) were cultured to produce multiple shoots (10-15 shoots/explant) on MS medium containing 0.1 mgl(-1) IAA and 2.5mgl(-1) BAP. In vitro produced shoots were cut into segments and cultured on shoot proliferation medium but with only 1.0 mgl(-1) of BAP to further multiply the shoots. Isolated individual shoots were cultured on a filter paper bridge in half strength MS liquid medium containing 25 mgl(-1) of IBA for 72 h in the dark at 28±2(0) C for induction of root(s). About 70-80 percent of shoots rooted. The treelets developed were hardened and transferred to pots. Around 20,000 plants can be obtained from a single explant within a period of 6 months. The protocol is highly reproducible and efficient.

摘要

已开发出一种体外方法，用于克隆和大量繁殖高度耐旱的印度沙漠树种 Maytenus emarginata。从一棵“加号”树（30 岁）上采集的茎段在含有 0.1 mgl(-1) IAA 和 2.5mgl(-1) BAP 的 MS 培养基上培养，以产生多个芽（每个外植体 10-15 个芽）。在离体产生的芽上切成段，并在芽增殖培养基上培养，但 BAP 仅为 1.0 mgl(-1)，以进一步增加芽的数量。将分离的单个芽在含有 25 mgl(-1) IBA 的半强度 MS 液体培养基中的滤纸桥上暗培养 72 h，在 28±2(0) C 下诱导生根。约 70-80%的芽生根。发育的小树经过硬化后转移到盆中。在 6 个月的时间内，每个外植体可获得约 20000 株植物。该方案具有高度的可重复性和效率。