Zaboronok Alexander, Isobe Tomonori, Yamamoto Tetsuya, Sato Eisuke, Takada Kenta, Sakae Takeji, Tsurushima Hideo, Matsumura Akira
Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
J Radiat Res. 2014 Mar 1;55(2):283-7. doi: 10.1093/jrr/rrt119. Epub 2013 Nov 1.
Migration and invasion of malignant glioma play a major role in tumor progression and can be increased by low doses of gamma or X-ray irradiation, especially when the migrated tumor cells are located at a distance from the main tumor mass or postoperative cavity and are irradiated in fractions. We studied the influence of proton beam irradiation on migration and invasion of human U87 malignant glioma (U87MG) cells. Irradiation at 4 and 8 Gy increased cell migration by 9.8% (±4, P = 0.032) and 11.6% (±6.6, P = 0.031) and invasion by 45.1% (±16.5, P = 0.04) and 40.5% (±12.7, P = 0.041), respectively. After irradiation at 2 and 16 Gy, cell motility did not differ from that at 0 Gy. We determined that an increase in proton beam irradiation dose to over 16 Gy might provide tumor growth control, although additional specific treatment might be necessary to prevent the potentially increased motility of glioma cells during proton beam therapy.
恶性胶质瘤的迁移和侵袭在肿瘤进展中起主要作用，低剂量的伽马射线或X射线照射可增加其迁移和侵袭，特别是当迁移的肿瘤细胞远离主要肿瘤块或术后腔并分次照射时。我们研究了质子束照射对人U87恶性胶质瘤（U87MG）细胞迁移和侵袭的影响。4 Gy和8 Gy照射分别使细胞迁移增加了9.8%（±4，P = 0.032）和11.6%（±6.6，P = 0.031），侵袭增加了45.1%（±16.5，P = 0.04）和40.5%（±12.7，P = 0.041）。2 Gy和16 Gy照射后，细胞运动性与0 Gy时无差异。我们确定，将质子束照射剂量增加到超过16 Gy可能会控制肿瘤生长，尽管可能需要额外的特定治疗来防止质子束治疗期间胶质瘤细胞潜在增加的运动性。
