Department of Radiotherapy, Tangdu Hospital, Fourth Military Medical University, Xinsi Road 1, Xi'an, Shaanxi, China.
World J Surg Oncol. 2013 Nov 5;11:288. doi: 10.1186/1477-7819-11-288.
X-chromosome-linked IAP (XIAP) and nuclear factor-κB (NF-κB) are frequently overexpressed and correlate closely with chemoradiotherapy resistance and poor prognosis in many cancers. However, the significance of XIAP and NF-κB expression in radiotherapy sensitivity and its effect on the prognosis of esophageal squamous cell carcinoma (ESCC) are still unknown. The aim of this study was to examine XIAP and NF-κB status in ESCC patients undergoing postoperative radiotherapy after radical surgery, and to evaluate their clinical significance.
A total of 78 ESCC patients treated with postoperative radiotherapy after radical surgery were enrolled in this study. We immunohistochemically investigated the expression of XIAP and NF-κB in tissues from enrolled patients with specific antibodies. Then, the correlations among XIAP, NF-κB expression, clinicopathological features and its prognostic relevance in ESCC were analyzed.
The increased expression of XIAP and NF-κB in ESCC tissues were clearly correlated with the tumor differentiation and p-TNM stage. Significant positive correlations were found between the expression status of XIAP and NF-κB (r = 0.779, P = 0.000). Overexpression of XIAP and NF-κB and metastasis were significantly associated with shorter overall survival times in univariate analysis (P < 0.05). Multivariate analysis also confirmed that XIAP expression was an independent prognostic factor (P = 0.005).
XIAP and NF-κB are intensively expressed in ESCC. The level of XIAP is positively correlated to progression and prognosis of ESCC.
X 染色体连锁凋亡抑制蛋白（XIAP）和核因子-κB（NF-κB）在许多癌症中经常过表达，与化疗和放疗抵抗以及不良预后密切相关。然而，XIAP 和 NF-κB 表达在放射敏感性中的意义及其对食管鳞状细胞癌（ESCC）预后的影响尚不清楚。本研究旨在检测接受根治性手术后接受术后放疗的 ESCC 患者的 XIAP 和 NF-κB 状态，并评估其临床意义。
本研究共纳入 78 例接受根治性手术后接受术后放疗的 ESCC 患者。我们使用特异性抗体通过免疫组织化学方法检测纳入患者组织中 XIAP 和 NF-κB 的表达。然后，分析了 XIAP、NF-κB 表达与临床病理特征之间的相关性及其在 ESCC 中的预后相关性。
XIAP 和 NF-κB 在 ESCC 组织中的表达增加与肿瘤分化和 p-TNM 分期明显相关。XIAP 和 NF-κB 的表达状态之间存在显著的正相关（r=0.779，P=0.000）。在单因素分析中，XIAP 和 NF-κB 的过表达和转移与总生存时间明显缩短相关（P<0.05）。多因素分析还证实 XIAP 表达是独立的预后因素（P=0.005）。
XIAP 和 NF-κB 在 ESCC 中表达强烈。XIAP 的水平与 ESCC 的进展和预后呈正相关。
