Peng Hai, Wang Li-Guo, Wang Xue-Zhi, Liu Ai-Jun
1st Department of Surgical Oncology, Chifeng Municipal Hospital, Chifeng 024000, PR China.
1st Department of Surgical Oncology, Chifeng Municipal Hospital, Chifeng 024000, PR China.
Pathol Res Pract. 2017 Dec;213(12):1482-1488. doi: 10.1016/j.prp.2017.10.008. Epub 2017 Oct 9.
This study aims to explore the correlation between X-linked inhibitor of apoptosis protein (XIAP) gene polymorphisms (rs8371 and rs9856) with the susceptibility and prognosis of esophageal squamous cell carcinoma (ESCC), providing a potential treatment for ESCC.
A total of 170 ESCC patients (case group) and 191 healthy people (control group) were enrolled in our study. Genotyping was conducted on the basis of the ligase detection reaction (LDR). The expressions of XIAP polymorphisms were detected. The patients were followed up every three months until death or the last follow-up day. The overall survival (OS) and progression free survival (PFS) were recorded by Kaplan-Meier survival curve, and the relationship between XIAP gene polymorphism and risk and prognosis of ESCC was assessed by Cox multivariate analysis.
TT+CT genotype and T allele frequencies of XIAP rs8371 and rs9856 in the case group were significantly lower compared to those of the control group (all P<0.05), suggesting that TT+CT genotype of XIAP rs8371 and rs9856 was associated with ESCC susceptibility. XIAP rs8371 and rs9856 polymorphisms were associated with tumor node metastasis (TNM) staging, depth of invasion and lymph node metastasis. The OS and PFS of TT+CT genotype carriers of rs8371 were longer than those of CC genotype carriers. Smoking, alcohol, TNM staging, depth of invasion, and lymph node metastasis were significantly associated with the OS and PFS in ESCC patients. Higher TNM staging, depth of invasion, and presence of lymph node metastasis were independent risk factors, while XIAP rs8371 was an independent protective factor for the prognosis of ESCC patients.
The present study demonstrates that XIAP rs8371 and rs9856 are associated with susceptibility to ESCC, and rs8371 polymorphisms might serve as an indicator for improved clinical efficacy and prognosis of ESCC patients.
本研究旨在探讨X连锁凋亡抑制蛋白（XIAP）基因多态性（rs8371和rs9856）与食管鳞状细胞癌（ESCC）易感性及预后的相关性，为ESCC提供潜在治疗方法。
本研究共纳入170例ESCC患者（病例组）和191例健康人（对照组）。基于连接酶检测反应（LDR）进行基因分型。检测XIAP多态性的表达。每三个月对患者进行随访，直至死亡或最后随访日。采用Kaplan-Meier生存曲线记录总生存期（OS）和无进展生存期（PFS），并通过Cox多因素分析评估XIAP基因多态性与ESCC风险及预后的关系。
病例组中XIAP rs8371和rs9856的TT + CT基因型及T等位基因频率显著低于对照组（均P < 0.05），提示XIAP rs8371和rs9856的TT + CT基因型与ESCC易感性相关。XIAP rs8371和rs9856多态性与肿瘤淋巴结转移（TNM）分期、浸润深度及淋巴结转移相关。rs8371的TT + CT基因型携带者的OS和PFS长于CC基因型携带者。吸烟、饮酒、TNM分期、浸润深度及淋巴结转移与ESCC患者的OS和PFS显著相关。较高的TNM分期、浸润深度及淋巴结转移是独立危险因素，而XIAP rs8371是ESCC患者预后的独立保护因素。
本研究表明XIAP rs8371和rs9856与ESCC易感性相关，且rs8371多态性可能作为改善ESCC患者临床疗效及预后的指标。
