Bianco P, Hayashi Y, Silvestrini G, Termine J D, Bonucci E
Calcif Tissue Int. 1985 Dec;37(6):684-6. doi: 10.1007/BF02554931.
Osteonectin and bone Gla-protein have been localized at the ultrastructural level in calf scapula bone tissue. Post-embedding-decalcified thin sections of paraformaldehyde-fixed and Araldite-embedded specimens of calf scapula were incubated with rabbit antisera to bovine osteonectin and bone Gla-protein, and the sites of antigen-antibody reaction were demonstrated using the Protein A-gold technique. Specific labelling of the bone matrix was obtained with both antisera. The collagen fibers were labelled particularly intensely. Filamentous structures visible at the sites of early mineralization were labelled by rows of gold particles after incubation with anti-osteonectin antiserum. These findings are consistent with biochemical data available on the localization and properties of these non- collagenous proteins. 'Crystal ghost'-like structures are immunoreactive to anti-osteonectin antiserum.
骨连接蛋白和骨钙素已在超微结构水平定位到小牛肩胛骨骨组织中。用兔抗牛骨连接蛋白和骨钙素抗血清孵育经多聚甲醛固定、环氧树脂包埋的小牛肩胛骨标本的包埋后脱钙薄片，并用蛋白A-金技术显示抗原-抗体反应位点。两种抗血清均获得了骨基质的特异性标记。胶原纤维的标记尤为强烈。用抗骨连接蛋白抗血清孵育后，早期矿化部位可见的丝状结构被一排金颗粒标记。这些发现与关于这些非胶原蛋白的定位和特性的生化数据一致。“晶体幽灵”样结构对抗骨连接蛋白抗血清具有免疫反应性。
