Vezenegho Samuel B, Chiphwanya John, Hunt Richard H, Coetzee Maureen, Bass Chris, Koekemoer Lizette L
Vector Control Reference Laboratory, Centre for Opportunistic, Tropical and Hospital Infections, National Institute for Communicable Diseases, National Health Laboratory Service, Sandringham, Johannesburg, South Africa.
Trans R Soc Trop Med Hyg. 2013 Dec;107(12):753-62. doi: 10.1093/trstmh/trt089. Epub 2013 Nov 4.
Limited information is available on malaria vector composition, feeding habits and malaria transmission in northern Malawi. Evidence of mosquito species diversity in this area was established in 2009, when Anopheles funestus-like, a new member of the An. funestus group was described. Additional biological information is needed to identify this species and to understand its role in malaria transmission.
Anopheline mosquitoes were collected in northern Malawi and analyzed for Plasmodium species infection, blood meal source and susceptibility to insecticides. A new hydrolysis probe assay was designed to identify An. funestus-like.
Anopheles funestus and An. rivulorum predominated in the indoor collections. Most An. funestus-like were collected indoors, mainly fed on animals and were uninfected with P. falciparum. Anopheles funestus showed insecticide resistance to deltamethrin and bendiocarb. A high-precision hydrolysis probe assay was successfully developed to identify An. funestus-like.
Four species in the An. funestus group were collected in Karonga. Resistance to deltamethrin and bendiocarb was observed in An. funestus and further investigation is needed on the insecticide resistance mechanisms. Anopheles funestus-like, while collected indoors, is mainly zoophilic and most likely not a malaria vector.
An. funestus (GenBank accession no. KC771136), An. funestus-like (GenBank accession no. KC771137), An. parensis GenBank accession no. KC771138) and An. vaneedeni GenBank accession no. KC771139).
关于马拉维北部疟疾媒介组成、摄食习性和疟疾传播的信息有限。2009年确定了该地区蚊子种类多样性的证据，当时描述了嗜人按蚊属的一个新成员——类嗜人按蚊。需要更多生物学信息来鉴定该物种并了解其在疟疾传播中的作用。
在马拉维北部采集按蚊，分析疟原虫种类感染情况、血餐来源和对杀虫剂的敏感性。设计了一种新的水解探针检测法来鉴定类嗜人按蚊。
嗜人按蚊和溪流按蚊在室内采集的样本中占主导地位。大多数类嗜人按蚊是在室内采集的，主要以动物为食，未感染恶性疟原虫。嗜人按蚊对溴氰菊酯和残杀威表现出抗药性。成功开发了一种高精度水解探针检测法来鉴定类嗜人按蚊。
在卡龙加采集到嗜人按蚊属的四个物种。在嗜人按蚊中观察到对溴氰菊酯和残杀威的抗药性，需要进一步研究其抗药机制。类嗜人按蚊虽然在室内采集到，但主要偏好吸食动物血液，很可能不是疟疾媒介。
嗜人按蚊（GenBank登录号KC771136）、类嗜人按蚊（GenBank登录号KC771137）、帕伦西斯按蚊（GenBank登录号KC771138）和瓦内登按蚊（GenBank登录号KC771139）。
