Department of Biological Sciences, University of Nevada, Las Vegas, 4505 Maryland Parkway, 89154, Las Vegas, Nevada, USA.
Microb Ecol. 1993 Sep;26(2):145-59. doi: 10.1007/BF00177049.
The distribution of aerobic chemoheterotrophic microorganisms within a 21 m3 section of deep subsurface rock was determined. Nineteen samples for microbiological analysis were aseptically taken by hand from the walls of a 400 m deep subsurface tunnel after an alpine miner created fresh rock faces 0.76, 1.52, 2.28, and 3.04 m into the tunnel wall. The direct counts were several orders of magnitude greater than viable counts in all samples. One of each morphologically distinct bacterial type from each sample was purified and analyzed for fatty acid methyl esters (FAME) using the Microbial Identification System (MIDI). Numbers of bacterial types, diversity, and equitability of recoverable microbial communities were the same or similar using either morphotype or FAME analyses as the basis for distinguishing between bacterial types. Twenty-nine genera (Euclidean distance of [Symbol: see text]25) were found within the rock section, while 28 of the 210 bacterial types isolated were nonculturable under the growth regime required for cluster analysis. Most isolates clustered at the genus level with Arthrobacter, Gordona, and Acinetobacter. Two genera, containing 16 isolates, were unmatched to known organisms within the MIDI data base and clustered with other isolates at a Euclidean distance greater than 50. While some species (Euclidean distance [Symbol: see text]10) were recovered from multiple sites within the rock section, most were found at 1-3 sites and usually without a definitive pattern of distribution.
对 21 立方米深部地下岩石段内好氧化能异养微生物的分布进行了确定。在高山矿工在隧道壁内新形成的新鲜岩石面 0.76、1.52、2.28 和 3.04 米深处，从 400 米深的地下隧道壁上无菌采集了 19 个微生物分析样品。直接计数在所有样品中均比活菌计数高出几个数量级。从每个样品中每种形态上明显不同的细菌类型中各纯化一个，并使用微生物鉴定系统（MIDI）分析脂肪酸甲酯（FAME）。使用形态或 FAME 分析作为区分细菌类型的基础，可得到相同或相似的细菌类型数量、可恢复微生物群落的多样性和均匀度。在岩石段内发现了 29 个属（欧几里得距离 [Symbol: see text]25），而在 210 种分离的细菌类型中，有 28 种在聚类分析所需的生长条件下不可培养。大多数分离株在属水平上与节杆菌属、戈登氏菌属和不动杆菌属聚类。有两个属，包含 16 个分离株，与 MIDI 数据库中已知的生物不匹配，并与其他分离株聚类，欧几里得距离大于 50。虽然一些物种（欧几里得距离 [Symbol: see text]10）从岩石段内的多个位置回收，但大多数在 1-3 个位置发现，并且通常没有明确的分布模式。
