Department of Biological Sciences, University of Nevada, Las Vegas, Las Vegas, Nevada 89154-4004.
Appl Environ Microbiol. 1993 Mar;59(3):933-5. doi: 10.1128/aem.59.3.933-935.1993.
Sets of bacterial isolates with the same colony morphologies were selected from spread plates of bacteria from deep subsurface rock samples; each set had a unique morphology. API-rapid-NFT analysis revealed that isolates within a set were the same. Fatty acid methyl ester analysis of one set of isolates clustered organisms within the same species, defining variation between isolates at the biotype (subspecies) and strain levels. Metal resistances consistently tracked with colony morphology, while antibiotic resistances were less reliable.
从深部地下岩石样本的细菌扩散平板中选择具有相同菌落形态的细菌分离株集落;每个集落都有独特的形态。API-rapid-NFT 分析表明,一个集落中的分离株是相同的。对一组分离株的脂肪酸甲酯分析将同一物种内的生物聚类在一起,确定了分离株在生物型(亚种)和菌株水平上的差异。金属抗性与菌落形态一致,而抗生素抗性则不太可靠。